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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MABD88 描述 Anti-SSEA-5 Antibody, clone 8e11 背景信息 Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures. 产品信息 格式 Purified 控制 - H9 Human Embryonic Stem Cells
演示 Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This SSEA-5 antibody is validated for use in Flow & ICC for the detection of the SSEA-5 protein. 主要应用 - Immunocytochemistry
- Flow Cytometry
应用说明 This antibody has been shown to work in Flow Cytometry applications (Weissman, I., et al. (2011).
This antibody has been shown to work in Immunohistochemistry applications (Weissman, I., et al. (2011).生物信息 免疫原品种 Undifferentiated H9 hESCs 克隆 8e11 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody 纯化方法 Protein G purified 产品使用声明 质量保证 Evaluated by Immunocytochemistry in H9 Human Embryonic Stem Cells.
Immunocytochemical Analysis: A 1:500 dilution of this antibody detected SSEA-5 in H9 Human Embryonic Stem Cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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