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  • merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit

  • merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit

    产品名称：CpG WIZ E-Cadherin Amplification Kit
    产品型号：S7804
    The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).

    merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit

  • 产品介绍
  • merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit

    描述
    产品目录编号 S7804
    品牌系列 Chemicon®
    商名
    • Chemicon
    • CpG Wiz
    描述 CpG WIZ® E-Cadherin Amplification Kit
    概述 E-cadherin is a cell adhesion protein that suppresses tumor cell invasion and metastasis in tumor models. On mechanism regulating the expression of the E-cadherin gene is methylation of the promoter region. The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated) for E-cadherin are also included.



    Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
    Alternate Names
    • CD324
    应用
    应用 The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).
    主要应用
    • PCR
    应用说明 Principles of the Technique

    Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® E-cadherin Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
    生物信息
    品种反应性 Human
    Entrez基因编号
    • NM_004360.2
    Entrez基因汇总 This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
    基因符号
    • LCAM
    • CDH1
    • CDHE
    • E-cadherin
    • Arc-1
    • Uvomorulin
    • UVO
    • Cadherin-1
    • CD324
    • uvomorulin
    • ECAD
    UniProt编号
    • P12830
    UniProt汇总 FUNCTION: SwissProt: P12830 # E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
    SIZE: 882 amino acids; 97456 Da
    SUBUNIT: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLG7.
    SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.
    TISSUE SPECIFICITY: Non-neural epithelial tissues.
    PTM: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.
    DISEASE: "SwissProt: P12830 # Defects in CDH1 are involved in dysfunction of the cell- cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. & Defects in CDH1 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. & Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089]."
    SIMILARITY: SwissProt: P12830 ## Contains 5 cadherin domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 25 assays

    merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit

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