- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,FCCH025104,FlowCellect™ Multi-Color DNA Damage Response Kit
产品名称：FlowCellect™ Multi-Color DNA Damage Response Kit
产品型号：FCCH025104
This FlowCellect Multi-Color DNA Damage Response Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of ATM, SMC1 & Histone H2A.X by flow c More>>
merck millipore,默克密理博,FCCH025104,FlowCellect™ Multi-Color DNA Damage Response Kit
- 产品介绍
描述 产品目录编号 FCCH025104 商名 - FlowCellect
描述 FlowCellect™ Multi-Color DNA Damage Response Kit 概述 Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of ATM, SMC1 and Histone H2A.X by flow cytometry. Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit was developed and tested using the DNA damaging reagent, Etoposide, in HeLa cells as a model system but the kit can be used with other human cell lines to determine the effect of mechanical and chemical reagents that can induce DNA damage through the ATM dependent pathway.
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit contains sufficient reagents for 25 3-color samples. The kit includes three optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.Alternate Names - Flow Cellect
背景信息 The signaling pathways by which cells respond to DNA damage is an important area of study for cancer research. The mammalian cell response to DNA damage is made up of highly coordinated signaling pathways that can initiate cell cycle arrest and repair or initiate apoptosis, depending on the extent and type of the damage. Cells that are defective in DNA damage signaling pathways can cause cancer because they lack the ability to sense and repair the damage, leading to genetic instability and ultimately uncontrolled cell growth.
The main kinase activated in response to double-stranded DNA breaks is ATM or Ataxia-telangiectasia mutated kinase. ATM is a member of the phospho inositide 3-kinase (PI3K)-related Ser/Thr protein kinase family. Inactive ATM exists as a dimer but quickly dissociates and becomes phosphorylated on Serine 1981 in response to ionizing radiation.
Once activated, ATM phosphorylates a number of downstream factors, including P53, CHK2, SMC1, NBS1 and Histone H2A.X. SMC1 is a member of the structural maintenance of chromosome family originally identified in yeast to be important for proper segregation of the chromosomes during mitosis. However, in mammalian cells exposed to IR, phosphorylation of SMC1 on sites S957 and S966 by ATM has been shown to be required for activation of the S-phase checkpoint and is important for protection against radiation induced cell death.
Phosphorylation of the histone variant, H2A.X, at serine 139 by ATM is an important indicator of DNA damage. As the level of DNA damage increases, the level of phospho Histone H2A.X increases and accumulates at the sites of DNA damage and is often used to indicate the level of DNA damage present within the cell (8). H2AX is also responsible for recruiting response proteins to the site of DNA damage.产品信息 部件 - 7. 20X Anti-pHistoneH2A.X(S139)-PerCP: (Part No. CS204852) 1 vial containing 150 µL
- 1. 10X Wash Buffer: (Part No. CS202123) 1 bottle containing 13 mL
- 2. Fixation Buffer: (Part No. CS202122) 1 bottle containing 13 mL
- 3. 5X Assay Buffer: (Part No. CS202124) 1 bottle containing 55 mL
- 4. 1X Permeabilization Buffer: (Part No. CS202125) 1 bottle containing 13 mL
- 5. 20X Anti-pSMC1(S957)-AlexaFluor®488: (Part No. CS204859) 1 vial containing 150 µL
- 6. 20X Anti-pATM(S1981)-PE: (Part No.CS204855) 1 vial containing 150 µL
检测方法 Fluorescent 应用 应用 This FlowCellect Multi-Color DNA Damage Response Kit for Flow Cytometry is designed to enable a researcher a quick & easy way to detect the phosphorylation state of ATM, SMC1 & Histone H2A.X by flow cytometry. 主要应用 - Flow Cytometry
生物信息 宿主 Mouse 品种反应性 Human 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 25 Tests / Kit
上一件merck millipore产品：merck millipore,默克密理博,05-143,Anti-PLA2 Antibody, secretory
下一件merck millipore产品：merck millipore,默克密理博,386032,Anti-Hsp70 Mouse mAb (C92F3A-5)
相关产品
merck millipore,默克密理博,SF-103,Scramble Control Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,S7804,CpG WIZ E-Cadherin Amplification Kit
merck millipore,默克密理博,492030,NS 2028 - CAS 204326-43-2 - Calbiochem
merck millipore,默克密理博,MABC688,Anti-RIP Antibody, clone EPR4689, rabbit monoclonal
merck millipore,默克密理博,05-143,Anti-PLA2 Antibody, secretory
merck millipore,默克密理博,386032,Anti-Hsp70 Mouse mAb (C92F3A-5)
merck millipore,默克密理博,AP183B,Goat Anti-Rat IgG Antibody, biotin-SP conjugate, Species Adsorbed
merck millipore,默克密理博,ELI-008-M,IL-6 ELISPOT Antibody Pair, Mouse