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merck millipore,默克密理博,71462,pET-48b(+) DNA - Novagen
产品名称：pET-48b(+) DNA - Novagen
产品型号：71462
Novagen's pET-48b(+) vector is designed for cloning and high-level expression of proteins fused with the 109aa Trx-Tag thioredoxin protein that is cleavable with the human rhinovirus 3C protease.
merck millipore,默克密理博,71462,pET-48b(+) DNA - Novagen
- 产品介绍
描述 概述 The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein (1) that is cleavable with the human rhinovirus (HRV) 3C protease (2). Cloning sites are available for producing fusion proteins also containing a cleavable N- terminal His•Tag® sequence for detection and purification. The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (3).
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.产品目录编号 71462 品牌系列 Novagen®
参考 参考 1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187-193.
2. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.
3. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.产品信息 融合标签 His•Tag, Trx•Tag, S•Tag 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
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