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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6004 Replaces AB815 描述 Anti-MT1-MMP Antibody, hinge region Alternate Names - membrane type 1 metalloprotease
- MT-MMP 1
- Membrane-type matrix metalloproteinase 1
- Membrane-type-1 matrix metalloproteinase
- matrix metallopeptidase 14 (membrane-inserted)
- matrix metalloproteinase 14
- matrix metalloproteinase 14 (membrane-inserted)
背景信息 Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade the components of extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis, cancer metastases, and tissue remodeling within many disease states.
Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.产品信息 格式 Affinity Purified 控制 - Rat lung lysate.
演示 Purified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3. 应用 应用 Detect MT1-MMP using this Anti-MT1-MMP Antibody, hinge region validated for use in WB, IH(P). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH conjugated synthetic peptide selected from the hinge region of human MT1-MMP. 表位 Hinge region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes human and rat MT1-MMP. It does not cross react with MMP-1, MMP-2, MMP-8, MMP-9, and MMP-13. 品种反应性 RatHuman Species Reactivity Note Human and rat. Predicted to cross react with mouse, (95% sequence homology) and monkey chimpanzee, canine and bovine (100% sequence homology). Reactivity with other species has not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - P50281
基因符号 - MTMMP1
- EC 3.4.24.80
- MMP-14
- MMP-X1
- MT1-MMP
- MT1MMP
纯化方法 Peptide affinity purified UniProt编号 - P50281
UniProt汇总 FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
CATALYTIC ACTIVITY: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
COFACTOR: Binds 1 zinc ion per subunit By similarity.
Calcium By similarity.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase By similarity.
SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated on a representative lot by Western blot on rat lung lysate using Anti-MT1-MMP. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 1 year from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,370660,Guanosine 3ʹ,5ʹ-cyclic Monophosphate, N²,2ʹ-O-Dibutyryl-, Sodium Salt - CAS 51116-00-8 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,539790,ProteoExtract Subcellular Proteome Extraction Kit
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