merck millipore,默克密理愽
  • merck millipore,默克密理博,S7115,ApopTag Positive Control Slides, rat mammary gland 4d post weaning

  • merck millipore,默克密理博,S7115,ApopTag Positive Control Slides, rat mammary gland 4d post weaning

    产品名称：ApopTag Positive Control Slides, rat mammary gland 4d post weaning
    产品型号：S7115
    This product is intended as a positive control for use with all ApopTag In Situ Apoptosis Detection Kits.

    merck millipore,默克密理博,S7115,ApopTag Positive Control Slides, rat mammary gland 4d post weaning

  • 产品介绍
  • merck millipore,默克密理博,S7115,ApopTag Positive Control Slides, rat mammary gland 4d post weaning

    重要规格表
    描述
    产品目录编号 S7115
    品牌系列 Chemicon®
    商名
    • Chemicon
    • ApopTag
    描述 ApopTag® Positive Control Slides, rat mammary gland 4d post weaning
    概述 This product is intended as a positive control for use with all ApopTag® In Situ Apoptosis Detection Kits. These reference slides are also useful as teaching aides, and for procedural troubleshooting. Each pack of ApopTag® Control Slides contains 5 unstained positive control slides. Rat mammary glands were obtained at the fourth day after weaning and were fixed for 18 hours in 10% neutral buffered formalin. After embedding in paraffin, 5 μm thick sections were cut from the middle of the tissue and were mounted on silanized slides.
    背景信息 Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
    应用
    应用 This product is intended as a positive control for use with all ApopTag In Situ Apoptosis Detection Kits.
    应用说明 Interpretation of Staining

    These positive control slides of female rat mammary tissue 4-days post weaning show only 1-2% of the cells being apoptotic. Microscopic inspection is usually required to confirm proper kit activity.

    Stain is generally localized to chromatin, and is intensely colored. The localization and sensitivity to be expected with the ApopTag® Peroxidase (S7100, S7101) or ApopTag® Fluorescein (S7110, S7111, S7160, S7165) Kits are similar. Positive objects vary in size, from intact nuclei to much smaller apoptotic bodies, and in shape, from round to irregular. Such objects may be either isolated, clustered, or engulfed in the cytoplasm of a phagocytic cell. Some unstained apoptotic nuclei or bodies may also be expected. Light cytoplasmic staining might co-localize with intense chromatin staining (this might be due to fragmented DNA leaching from the nucleus, or that in phagocytic organelles). If staining of non-apoptotic cell nuclei is observed with the ApopTag® Peroxidase kits (S7100, S7101), the user can attempt to decrease the substrate development time or temperature, or to further dilute the anti-digoxigenin antibody reagent in 0.5% (w:v) BSA. Counterstaining is recommended (see The Complete ApopTag® Manual).



    Limitations

    This control tissue has been selected for positive staining with all ApopTag® Kits. Results with control tissue may vary from results with other tissues because of both sample preparation differences and intrinsic tissue differences.
    产品使用声明
    质量保证 ApopTag® Control Slides have been qualified for use with all ApopTag® Kits.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Recommended Storage 18°C to 25°C.
    包装信息
    数量 5 ea

    merck millipore,默克密理博,S7115,ApopTag Positive Control Slides, rat mammary gland 4d post weaning

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