merck millipore,默克密理博,AB1778,Anti-Calbindin D-28K Antibody

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merck millipore,默克密理博,AB1778,Anti-Calbindin D-28K Antibody
产品名称：Anti-Calbindin D-28K Antibody
产品型号：AB1778
This Anti-Calbindin D-28K Antibody is validated for use in IC, IH, IH(P), WB for the detection of Calbindin D-28K.

merck millipore,默克密理博,AB1778,Anti-Calbindin D-28K Antibody

产品介绍

merck millipore,默克密理博,AB1778,Anti-Calbindin D-28K Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC, IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB1778
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Calbindin D-28K Antibody
Alternate Names	calbindin 1, 28kDa Calbindin D28 RTVL-H protein Vitamin D-dependent calcium-binding protein, avian-type 3 calbindin calbindin 1, (28kD) calbindin 1 (28kD)
背景信息	Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. The biological roles of Calbindin D-28k include, calcium regulation (including calcium transport and uptake, calcification of bone and teeth) and calcium related signalling in neurons and transiently in embryological development. This calbindin also has a role in protecting neurons from apoptotic cell death. Disregulation of this calbindin protein relates to several disease states. Decline in calbindin D-28k in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28k immunoreactivity is specifically absent from neuronal poplulations lost early in ALS, while substantial loss of neurons which do contain the protein occur in patients with Huntington's disease.

产品信息
格式	Affinity Purified
控制	Mouse brain lysates and rat small intestine lysates.
演示	Purified rabbit polyclonal in phosphate buffered saline containing an antibody that has been affinity preabsorbed against calretinin. Contains no preservative.

应用
应用	This Anti-Calbindin D-28K Antibody is validated for use in IC, IH, IH(P), WB for the detection of Calbindin D-28K.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunocytochemistry: 1:1000-1:5000. See online protocols. Immunohistochemistry(paraffin): Optimal working dilutions and protocols must be determined by the end user.

生物信息
免疫原品种	Recombinant mouse calbindin.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Calbindin. Shows no cross-reactivity to calretinin by Western blot. The antibody produces specific staining of cerebellum Purkinje cells, molecular layer dendrites and axonal fibers, stains cell bodies and fibers in neuronal subpopulations.
品种反应性	RatHumanMouse
Species Reactivity Note	Mouse. Due to the homology of the protein it is expected to react with human, rat, and most species.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_004929.2
Entrez基因汇总	Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted.
基因符号	D-28K CAB27 CALB
纯化方法	Affinity Purfied
UniProt编号	P05937
UniProt汇总	FUNCTION: SwissProt: P05937 # Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. SIZE: 261 amino acids; 30025 Da SUBUNIT: Interacts with RANBP9. DOMAIN: SwissProt: P05937 This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. SIMILARITY: Belongs to the calbindin family. & Contains 5 EF-hand domains.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected CALBINDIN on 10 μg of Mouse Brain lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

储存和货运信息

存储条件

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.