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检测方法 Colorimetric 描述 概述 A highly sensitive and specific kit for detection of MMP-13 from human samples, including serum, synovial fluid, and cell culture supernatant. Recognizes both the latent and active forms of MMP-13, however exhibits 10-fold higher sensitivity towards the active than the latent form of MMP-13. Does not recognize MMP-1, MMP-2, MMP-3, MMP-8, or MMP-9. 产品目录编号 QIA130 品牌系列 Calbiochem®
同义词 Collagenase-3 ELISA Kit, Matrix Metalloproteinase ELISA Kit 参考 参考 Bluteau G., et al. 2001. Biochim. Biophys. Acta 1526, 147.
Brew, K., et al. 2000. Biochim Biophys. Acta 1477, 267.
Pendas A.M., et al. 2000. Clin. Chim. Acta 291, 137.
Nagase, H., and Woessner, F. Jr. 1999. J. Biol. Chem. 274, 21491.
Westhoff C.S., et al. 1999. Arthritis Rheum. 42, 1517.
Knauper V., et al. 1996. J. Biol. Chem. 271, 17124.产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 Coated 96-Well Plate, Assay Buffer, Wash Buffer Concentrate, Standard, Serum Standard Diluent, Detection Buffer, Biotinylated Antibody, Conjugate Solution, TMB Substrate, Stop Solution, and a user protocol. 生物信息 化验范围 32 - 2000 pg/ml 化验时间 ~5 hours 样品类型 Serum, synovial fluid, tissue culture supernatant 品种 human 理化信息 灵敏度 7 pg/ml 产品使用声明 预定用途 The MMP-13 ELISA Kit is a non-isotopic immunoassay for the in vitro quantitation of latent and active human MMP-13 protein in body fluids such as serum and synovial fluids. Note: When analyzing pro-MMP-13 and active MMP-13 in parallel, the level of pro-MMP-13 must be ~10 times higher than the level of active MMP-13 in order to obtain a comparable signal. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 All components of the kit can be stored in the refrigerator (4°C). Once reconstituted, the standard solution should be used immediately or stored at -20°C. The diluted Biotinylated Detection Antibody and diluted Conjugate Solution should be prepared freshly directly before use. When running a partial plate, only a suitable aliquot should be activated. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Coated 96-Well Plate, Assay Buffer, Wash Buffer Concentrate, Standard, Serum Standard Diluent, Detection Buffer, Biotinylated Antibody, Conjugate Solution, TMB Substrate, Stop Solution, and a user protocol.
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