merck millipore,默克密理博,04-1147,Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal

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merck millipore,默克密理博,04-1147,Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal
产品名称：Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal
产品型号：04-1147
This Anti-MCAM [CD146] Antibody, clone EPR3208, Rabbit is validated for use in WB, FC, IP, IH(P), IC for the detection of MCAM [CD146].

merck millipore,默克密理博,04-1147,Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1147,Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB, FC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-1147
描述	Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal
Alternate Names	melanoma cell adhesion molecule CD146 antigen Cell surface glycoprotein P1H12 Melanoma-associated antigen A32 Melanoma-associated antigen MUC18 S-endo 1 endothelial-associated antigen melanoma adhesion molecule
背景信息	MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.

产品信息
格式	Culture Supernatant
控制	A375 cells, fetal artery, and HUVEC lysates.
演示	Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	This Anti-MCAM [CD146] Antibody, clone EPR3208, Rabbit is validated for use in WB, FC, IP, IH(P), IC for the detection of MCAM [CD146].
主要应用	Immunocytochemistry Western Blotting Flow Cytometry
应用说明	Immunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected MCAM in melanoma tissue. Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot was used on IC. Immunoprecipitation: 1:10 dilution from a previous lot was used on IP. Flow Cytometry: 1:80 dilution from a previous lot was used on FC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in human MCAM.
克隆	EPR3208
宿主	Rabbit
特异性	This antibody recognizes MCAM.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P43121
基因符号	MUC18 CD146 Gicerin
纯化方法	Unpurified
UniProt编号	P43121
UniProt汇总	FUNCTION: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. SEQUENCE SIMILARITIES: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains.

产品使用声明
质量保证	Evaluated by Western Blot on A375 cell lysate, fetal artery, and HUVEC lysate. Western Blot Analysis: 1:10,000 dilution of this antibody was used to detect MCAM in A375 cell lysate, fetal artery, and HUVEC lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

包装信息
数量	100 µL

merck millipore,默克密理博,04-1147,Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal

上一件merck millipore产品：merck millipore,默克密理博,09-871,Anti-Elp1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,14-316,Blk Protein, active, 10 µg

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