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检测方法 Colorimetric 描述 概述 BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform. Note: 1 T = 1 test. 产品目录编号 QIA58 品牌系列 Calbiochem®
同义词 Bromodeoxyuridine Assay 参考 参考 Hardonk, M.J. and Harms, G. 1990. Acta histochemica, Suppl. 39, 99.
Muir, D., et al. 1990. Analytical Biochemistry 185, 377.
Lanier, T. L., et al. 1989. Carcinogenesis 10, 1341.
Magaud, J.-P., et al. 1988. J. Immunol. Methods 106, 95.
Collins, S.J. 1987. Blood 70, 1233.
Porstmann, T., et al. 1985. J. Immunol. Methods 82, 169.
Raza, A., et al. 1985. Cytometry 6, 633.
Morstyn, G., et al. 1983. J. Clin. Invest. 72, 1844.
Gratzner, H.G. 1982. Science 218, 474.产品信息 检测方法 Colorimetric 形式 200 or 1000 Tests 格式 96-well plate 试剂盒包含 BrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol. 生物信息 化验时间 3 h 样品类型 Intact Cells 安全信息 R相 R: 11-20/21/22-35-36/38-46-61
Highly flammable.
Harmful by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes and skin.
May cause heritable genetic damage.
May cause harm to the unborn child.S相 S: 7-16-26-36/37/39-45
Keep container tightly closed.
Keep away from sources of ignition - No Smoking.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® BrdU Cell Proliferation Assay is a non-isotopic immunoassay for the quantitation of bromodeoxyuridine incorporation into newly synthesized DNA of actively proliferating cells. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival store the entire contents pf the kit at -20°C, in a non-frostfree freezer.
Prior to use:
1. Remove the Fixative/Denaturing Solution and place at room temperature for at least 4 h prior to use. Precipitates that may occur while cold should go back into solution.
2. Reconstitute the Peroxidase Goat Anti-Mouse IgG with the appropriate volume of 1X PBS.
• For the 200 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 125 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
• For the 1000 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 250 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
Allow solution to stand at room temperature for 10 min. (or until solution is clear). Divide into small aliquots; aliquots not to be used that same day should be stored at -20°C in a non-frostfree freezer.
Once the kit components have been thawed for use:
• The BrdU label and 100X Anti-BrdU Antibody should be divided into small aliquots and stored at -20°C in a non-frostfree freezer with the Peroxidase Goat Anti-Mouse IgG; avoid multiple freeze/thaw cycles.
• All the other kit components (Substrate, Antibody Diluent, Conjugate Diluent, 20X Plate Wash Concentrate, Fixative/Denaturing Solution, Stop Solution) should be stored at 4°C.避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 BrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
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