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merck millipore,默克密理博,PS1023,PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)
产品名称:PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)
产品型号:PS1023
This PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).
merck millipore,默克密理博,PS1023,PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1. 产品目录编号 PS1023 品牌系列 Calbiochem®
应用数据 
Detection of human Smad3, phosphorylated at Ser423/425, by immunoblotting. Samples: whole cell lysate from HT-1080 cells, left untreated or treated with TGF-β1, TGFR inhibitor (SB-431542), or BMP-2, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) (Cat. No. PS1023) (1:1000) (top panel) and Anti-Smad3 (total) (bottom panel). Detection: chemiluminescence.参考 参考 Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.产品信息 形式 Liquid 配方 In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5. 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH. 克隆 C25A9 宿主 Rabbit 同种型 IgG 品种 human 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Do not aliquot.
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