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merck millipore,默克密理博,PRCYT2MAG40K,MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay
产品名称：MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay
产品型号：PRCYT2MAG40K
The analytes available for this multiplex kit are: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, More>>
merck millipore,默克密理博,PRCYT2MAG40K,MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,PRCYT2MAG40K,MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 TNFβ/LTA, sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5 NhP Mplex Luminex xMAP 描述 产品目录编号 PRCYT2MAG40K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay 概述 To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing
Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel 2 enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.背景信息 Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer. 产品信息 部件 - Mixing Bottle, 1 bottle
- Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized
- Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized
- Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized
- Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized
- Sample Plate, 96 well format, 1 plate
- 2 Foil plate sealers
- Assay Buffer, 2 bottles, 30 mL
- Bead Diluent, 1 bottle, 3.5 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
- Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL
- Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度（%） - Intra-assay: <10
- Inter-assay: <15
板面类型 MAGNETIC Cytokines/Chemokines 应用 应用 The analytes available for this multiplex kit are: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA. 主要应用 - Multiplexing
应用说明 • This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of 1:2 diluted plasma or serum or 25 μL cell culture supernatant per well.
• RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs.生物信息 特异性 Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. 品种反应性 Non-human Primate 可用的分析物 - TNFβ/LTA
- sCD137
- Eotaxin/CCL11
- sFasL
- FGF-2
- Fractalkine/CX3CL1
- Granzyme A
- Granzyme B
- IL-1α
- IL-2
- IL-4
- IL-6
- IL-16
- IL-17A
- IL-17E/IL-25
- IL-21
- IL-22
- IL-23
- IL-28A
- IL-31
- IL-33
- IP-10/CXCL10
- MIP-3α/CCL20
- Perforin
- RANTES/CCL5
化验面板 Everything you need in a single kit. 交叉反应性 This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details. 准确度 - 2 Hour Incubation: 74% - 95%
- Overnight Incubation: 80% - 109%
理化信息 灵敏度 - Refer to kit protocol for sensitivities for individual cytokines/chemokines
稀释的线性 Contact technical support for this information 标准曲线范围 - IL-17E/IL-25: 293 – 300,000 pg/mL
- RANTES/CCL5 (tissue culture): 1 – 750 pg/mL
- IL-17A: 1 – 1,500 pg/mL
- Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL
- Granzyme B: 4 – 4,000 pg/mL
- MIP-3α/CCL20: 7 – 7,000 pg/mL
- sFasL: 8 – 8,000 pg/mL
- sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL
- IL-21, IL-31: 15 – 15,000 pg/mL
- FGF-2, IL-33: 20 – 20,000 pg/mL
- IL-23: 24 – 25,000 pg/mL
- IL-1α, IL-28A: 29 – 30,000 pg/mL
- IL-16: 73 – 75,000 pg/mL
- Granzyme A: 78 – 80,000 pg/mL
- Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL
- IL-4: 146 – 150,000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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