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描述 概述 Recombinant, mouse ADAM10 (mature, active mouse ADAM10 ectodomain; amino acids 19-673) fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. An integral membrane proteinase involved in notch signaling. Also considered as an α-secretase candidate. 产品目录编号 PF124 品牌系列 Calbiochem®
同义词 Kuzbanian Metalloproteinase 参考 参考 Colciaghi, F., et al. 2002. Mol. Med. 8, 67.
Yan, Y., et al. 2002. J. Cell Biol. 158, 221.产品信息 活性 ≥5 pmol/min/µg as measured by the cleavage of 10 µM of the fluorescent substrate MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂ (Cat. No. 616402) by 100 ng of the enzyme in 100 µl of reaction buffer at 37°C. 形式 Lyophilized 配方 Lyophilized from a filtered solution of 100 mM NaCl, 25 mM MES, 10 µM ZnCl₂, 0.02% BRIJ®-35 Detergent, pH 5.5. Molar Mass 60000 应用 主要应用 Immunoblotting (Western Blotting)
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生物信息 纯度 ≥90% by SDS-PAGE 特异活性 ≥5 pmol/min/µg 理化信息 污染物 Endotoxin: ≤1 EU/µg ADAM10 溶解度 25 mM Tris-HCl buffer, pH 7.5 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C and up to 3 months at -70°C.
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