merck millipore,默克密理愽
  • merck millipore,默克密理博,NC011,Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

  • merck millipore,默克密理博,NC011,Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

    产品名称：Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands
    产品型号：NC011
    The murine 2.5S Nerve Growth Factor (or NGF protein) is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.

    merck millipore,默克密理博,NC011,Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

  • 产品介绍
  • merck millipore,默克密理博,NC011,Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

    重要规格表

    品种 来源 纯度
    MouseMouse submaxillary gland.Greater than 90% determined by SDS-PAGE electrophoresis.
    描述
    产品目录编号 NC011
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands
    概述 MOLECULAR WT.:

    13,000 reduced
    Alternate Names
    • beta-NGF
    背景信息 Murine 2.5S Nerve Growth Factor is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.. The 2.5S form of murine Nerve Growth Factor originates from the dissociation and proteolytic cleavage of the beta subunit of 7S forms of this neurotrophic factor and is also referred to as beta-NGF. This form mediates phosphorylation of specific intracellular proteins and is solely responsible for the biological activity of both the 2.5S and 7S forms of this neurotrophic factor. Murine 2.5S Nerve Growth Factor is expressed in sympathetic and sensory-innervated peripheral tissues such as the vas deferens, heart, iris, skin, splenic capsule, sciatic nerve and submaxillary gland. At the cellular level, mNGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. There is a strong correlation between the levels of mNGF mRNA in peripheral tissues and the density of sympathetic innervation. Peripheral neurons that respond to mNGF include adrenergic sympathetic neurons and primary sensory neurons. Cellular responses to this neurotrophic factor include differentiation, neurotic extension and production of catecholamine and neuropeptide-synthesizing enzymes. Murine Nerve Growth Factor binds the "low affinity" NGF receptor (p75NTR), as do all neurotrophic factors, and it mediates, via p75NTR signal transduction, the functional response of Schwann cells. mNGF also activates signal transduction by the dimerization and autophosphorylation of the TrkA receptor (also known as Trk and p140trk).
    产品信息
    演示 Lyophilized. Reconstitute to a concentration of 10 μg/mL with sterile DMEM. Medium with mNGF cannot be reused due to mNGF degradation.
    应用
    应用 The murine 2.5S Nerve Growth Factor (or NGF protein) is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.
    生物信息
    浓缩 1 mg/mL
    纯度 Greater than 90% determined by SDS-PAGE electrophoresis.
    来源 Mouse submaxillary gland.
    品种 Mouse
    特异活性 BIOLOGICAL ASSAY: <br /><br />This lot was bioassayed for neurotrophic activity using rat PC12 cell cultures over a period of 7-14 days. The activity is in the range of 0.5-50 ng/mL. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50 ng/mL of medium (Bradshaw, 1978).
    基因符号
    • NGFB
    • NGF
    UniProt编号
    • P01139
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain frozen at -70°C in undiluted aliquots for 4-6 weeks. Avoid repeated freeze-thaw cycles as it may result in a loss of activity.
    包装信息
    数量 50 µg
    包装 2x25 μg

    merck millipore,默克密理博,NC011,Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

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