merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB16985,Anti-MCAM Antibody, clone P1H12

  • merck millipore,默克密理博,MAB16985,Anti-MCAM Antibody, clone P1H12

    产品名称：Anti-MCAM Antibody, clone P1H12
    产品型号：MAB16985
    Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.

    merck millipore,默克密理博,MAB16985,Anti-MCAM Antibody, clone P1H12

  • 产品介绍
  • merck millipore,默克密理博,MAB16985,Anti-MCAM Antibody, clone P1H12

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, Ca, HWB, ELISA, FC, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB16985
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MCAM Antibody, clone P1H12
    Alternate Names
    • melanoma cell adhesion molecule
    • melanoma adhesion molecule
    • S-endo 1 endothelial-associated antigen
    • Melanoma-associated antigen MUC18
    • Melanoma-associated antigen A32
    • Cell surface glycoprotein P1H12
    • CD146 antigen
    背景信息 MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
    产品信息
    格式 Purified
    控制
    • HUVEC cells.
    演示 Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.
    应用
    应用 Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    不建议的应用
    • Immunohistochemistry (Paraffin)
    应用说明 Immunocytochemistry:
    1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

    Immunohistochemistry:
    1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

    Immunoprecipitation:
    1-10 μg/mL of a previous lot worked in immunoprecipitation.

    ELISA:
    1-10 μg/mL of a previous lot worked in ELISA.

    FACS Analysis:
    1-10 μg/mL of a previous lot worked in FACS.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Immunization of mice with HUVEC.
    克隆 P1H12
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation.
    同种型 IgG1
    品种反应性 MouseCanineHuman
    Species Reactivity Note Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_006500.2
    基因符号
    • CD146
    • MCAM
    • Gicerin
    • MUC18
    非反应性品种
    • Rat
    纯化方法 Protein A purfied
    UniProt编号
    • P43121
    UniProt汇总 FUNCTION: SwissProt: P43121 # Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
    SIZE: 646 amino acids; 71607 Da
    SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
    TISSUE SPECIFICITY: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
    SIMILARITY: SwissProt: P43121 ## Contains 3 Ig-like C2-type (immunoglobulin-like) domains. & Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on HUVEC lysates.

    Western Blot Analysis:
    1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB16985,Anti-MCAM Antibody, clone P1H12

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