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品种反应性 主要应用 宿主 格式 抗体类型 R, M, H, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS54 描述 Anti-AS160 Antibody Alternate Names - TBC1 domain family, member
- Acrg embryonic lethality minimal region ortholog
- Akt substrate of 160 kDa
- TBC (Tre-2, BUB2, CDC16) domain-containing protein
背景信息 AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2. 产品信息 格式 Affinity Purified 控制 - HEPG2 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected AS160 on 10 µg of HEPG2 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes AS160 at the C-terminus. 品种反应性 RatMouseHumanCanine Species Reactivity Note Demonstrated to react with human and mouse. Predicted to react on canine and rat based on 100% sequence homology.
Chimpanzee, equine, and ox (90% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001074747
基因符号 - AS160
纯化方法 Affinity Purfied UniProt编号 - Q8BYJ6
UniProt汇总 FUNCTION: May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Note: Cytoplasmic perinuclear (by similarity).
TISSUE SPECIFICTY: Widely expressed, including in pancreatic beta cells.
PTM: Phosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for GLUT4 translocation. Has no effect on GLUT4 internalization.
SEQUENCE SIMILARITIES: Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain.产品使用声明 质量保证 Evaluated by Western Blot in HEPG2 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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