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merck millipore,默克密理博,MCH200109,Muse™Autophagy LC3-antibody based Kit
产品名称：Muse™Autophagy LC3-antibody based Kit
产品型号：MCH200109
The Muse Autophagy LC3-antibody based kit provides a quantitative solution for the study of autophagy.
merck millipore,默克密理博,MCH200109,Muse™Autophagy LC3-antibody based Kit
- 产品介绍
描述 产品目录编号 MCH200109 商名 - Muse
描述 Muse™Autophagy LC3-antibody based Kit 概述 EMD Millipore’s Muse™ Autophagy LC3-antibody based kit provides a quantitative solution for the study of autophagy. This kit contains two key detection reagents to help facilitate the monitoring of lipidated LC3-II in a given cell system:
1) The use of selective permeabilization solution discriminates between cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins, while protecting LC3 which has been sequestered into the autophagosome;
2) Since autophagy is a constitutive cellular degradation process, the use of an autophagy detection reagent (Autophagy Reagent A) will prevent the lysosomal degradation of LC3, allowing its quantification by flow cytometry. EMD Millipore’s Muse™ Autophagy LC3-antibody based kit includes an anti-LC3 mouse monoclonal antibody conjugated to Alexa Fluor®555, used to measure and track the levels of LC3 within the cell. The anti-LC3 Alexa Fluor®555 conjugated antibody and autophagy enabling reagents have been carefully evaluated to ensure optimal performance, alleviating the need for any additional validation of the kit reagents. This kit contains reagents A and B, along with an assay buffer to provide researchers a complete solution for autophagy analysis.The autophagy detection reagents and antibody have been optimized together to ensure the ability to measure and discriminate between cytosolic and lipidated LC3 to accurately measure the autophagic process. Data generated using the Muse™ Cell Analyzer along with the corresponding Muse™ software module provides statistical values measuring:
• Mean Autophagy Value (for both control and test samples)
• Autophagy Induction Ratio (Test sample fluorescence relative to control)
• Percentage of cells with increased autophagy (Test sample versus control)
The Muse™ Autophagy LC3-antibody based kit is optimized on the Muse™ Cell Analyzer. The Muse™ System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse™ Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 50 tests.
For Research Use Only; not for use in diagnostic procedures.应用 应用 The Muse® Autophagy LC3-antibody based kit provides a quantitative solution for the study of autophagy. 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 100 Tests
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