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品种反应性 主要应用 宿主 格式 抗体类型 M ChIP, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 09-820 描述 Anti-Klf2 Antibody Alternate Names - Kruppel-like factor LKLF
- Kruppel-like factor 2 (lung)
- Lung krueppel-like factor
- krueppel-like factor 2
- lung Kruppel-like zinc finger transcription factor
背景信息 Krueppel-like factor 2 (Klf2) is a novel protein belonging to the KLF family of transcription factors, a subfamily of approximately 20 members characterized by their possession of a zinc-finger domain and functioning as transcriptional regulators for cellular differentiation and growth. Klf2 activates transcription through its binding of the beta-globin gene promoter CACCC box. Studies have shown Klf2 to have critical roles in both the function and differentiation of several cell types including; adipocytes, lung cells, T lymphocytes, and monocytes. It has also been newly identified as an endothelial transcription regulator for pro-inflammatory activation, and is a requirement for the migration of smooth muscle cells and normal vessel formation. Shear stress and pro-inflammatory cytokines are considered to be potent inhibitors of Klf2. Expression is primarily observed in lung and spleen tissue, but it has also been found in aortic tissue. 产品信息 格式 Serum 控制 - Mouse embryonic stem cell chromatin extract
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Use Anti-Klf2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Klf2 also known as Kruppel-like factor 2 (lung), Lung krueppel-like factor. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Klf2 from 2 x 10E6 to 1 x 10E7 mouse embryonic stem cells. (Huck Ng, Genome Institute of Singapore.) 生物信息 免疫原品种 GST-fusion protein with histidine-tagged mouse Klf2. 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Mouse Species Reactivity Note Demonstrated to react with mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_032478
基因符号 - LKLF
- Klf2
纯化方法 Unpurified UniProt编号 - Q60843
UniProt汇总 FUNCTION: Binds to the CACCC box in the beta-globin gene promoter and activates transcription.
SUBUNIT STRUCTURE: Interacts with WWP1 (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICTY: Predominant expression in the lungs and spleen.
PTM: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
SEQUENCE SIMILARITIES: Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.产品使用声明 质量保证 Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Klf2 in 10 µg of mouse embryonic stem cell chromatin extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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