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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9080 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Ryanodine Receptor 2 Antibody 产品信息 格式 Serum 控制 - Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
演示 Rabbit serum. Liquid. 应用 应用 Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the variant TM region of human Ryanodine Receptor 2. 宿主 Rabbit 特异性 Ryanodine Receptor 2. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001035.2
Entrez基因汇总 This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. 基因符号 - VTSIP
- RYR2
- hRYR-2
- ARVD2
- RyR2
- ARVC2
- RYR-2
UniProt编号 - Q92736
UniProt汇总 FUNCTION: SwissProt: Q92736 # Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T- tubules (By similarity).
SIZE: 4967 amino acids; 564498 Da
SUBUNIT: Homotetramer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
TISSUE SPECIFICITY: Heart muscle, brain (cerebellum and hippocampus) and placenta.
DEVELOPMENTAL STAGE: Expressed in myometrium during pregnancy.
DISEASE: SwissProt: Q92736 # Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia 2 (ARVD2) [MIM:600996]; also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. & Defects in RYR2 are the cause of an autosomal dominant form of stress-induced polymorphic ventricular tachycardia (VTSIP) [MIM:604772]; also known as catecholaminergic polymorphic ventricular tachycardia (CPVT). VTSIP is a genetic arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. & Defects in RYR2 are a cause of familial polymorphic ventricular tachycardia (FPVT) [MIM:192605]. FPVT is an autosomal- dominant, inherited disease with a relatively early onset and a mortality rate of approximately 30% by the age of 30 years. Phenotypically, it is characterized by salvoes of bidirectional and polymorphic ventricular tachycardias in response to vigorous exercise, with no structural evidence of myocardial disease.
SIMILARITY: SwissProt: Q92736 ## Belongs to the ryanodine receptor family. & Contains 3 B30.2/SPRY domains. & Contains 1 EF-hand domain. & Contains 5 MIR domains.
MISCELLANEOUS: The calcium release channel is modulated by calcium ions, magnesium ions, ATP and calmodulin. & The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the SR and the T-tubule. It is possible that the foot structure interacts with the cytoplasmic region of the dihydropyridine receptor. & Ryanodine is an alkaloid that binds to the Ca- release channel in junctional SR and modulates its activity.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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