merck millipore,默克密理博,MABN140,Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, M	IHC, ICC, WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	MABN140
描述	Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4
背景信息	NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

产品信息
格式	Purified
控制	Mouse brain nuclear extract
演示	Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4 detects level of NeuN (rabbit monoclonal) & has been published & validated for use in ICC, Western Blotting & IHC.
主要应用	Immunohistochemistry Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex and hippocampus primary cultures. Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected NeuN in mouse cerebellum and hippocampus tissues.

生物信息
免疫原品种	Recombinant protein corresponding to mouse NeuN.
克隆	27-4
宿主	Rabbit
同种型	IgG
品种反应性	RatMouse
Species Reactivity Note	Demonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology.
抗体类型	Monoclonal Antibody
纯化方法	Protein A purified

产品使用声明
质量保证	Evaluated by Western Blot in mouse brain nuclear extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MABN140,Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

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