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merck millipore,默克密理博,MABF260,Anti-NLRC5 Antibody, clone 3H8
产品名称：Anti-NLRC5 Antibody, clone 3H8
产品型号：MABF260
Detect NLRC5 using this rat monoclonal antibody, Anti-NLRC5 Antibody, clone 3H8 validated for use in western blotting, IP & Flow Cytometry.
merck millipore,默克密理博,MABF260,Anti-NLRC5 Antibody, clone 3H8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB R Protein G Purified Monoclonal Antibody 描述 产品目录编号 MABF260 描述 Anti-NLRC5 Antibody, clone 3H8 Alternate Names - Nucleotide-binding oligomerization domain protein 4
- Protein NLRC5
- Caterpiller protein 16.1
- CLR16.1
- Nucleotide-binding oligomerization domain protein 27
背景信息 The protein named NLRC5, or also known as Nucleotide-binding oligomerization domain protein 27, Caterpiller protein 16.1 (CLR16.1), or Nucleotide-binding oligomerization domain protein 4 and encoded by the gene NLRC5/NOD27/NOD4, plays a role in the homeostatic control of innate immunity and anti-viral defense mechanisms. NLRC5 also likely regulates the NF kappa B and type 1 interferon signaling response pathways induced upon infection. NLRC5 interacts with CHUK and IKBKB and prevents CHUK and IKBKB phosphorylation thus inhibiting their kinase activity. NLRC5 interacts with DDX58 and IFIH1 proteins and blocks the interaction of MAVS to DDX58 proteins. NLRC5 is localized to the cytoplasm. NLRC5 is expressed in spleen, thymus, lung, brain, tonsil, heart and prostate at moderate levels, but NLRC5 expression is dramatically induced by interferons during pathological infections. Traditional immune responses studies have demonstrated that NLRC5 is a specific and master regulator of major histocompatibility complex (MHC) class I genes as well as related genes involved in MHC class I antigen presentation. The expression of MHC class I genes is regulated by NLRC5 in coordination with the RFX components through an enhanceosome-dependent manner, and the involvement of NLRC5 in MHC class I mediated CD8+ T cell activation, proliferation and cytotoxicity has proved to be critical for host defense against intracellular bacterial infections. 产品信息 格式 Protein G Purified 演示 Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect NLRC5 using this rat monoclonal antibody, Anti-NLRC5 Antibody, clone 3H8 validated for use in western blotting, IP & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
应用说明 Flow Cytometry Analysis: 0.25 µg from a representative lot detected NLRC5 in 1X10E6 Jurkat and Raji cells.
Western Blotting Analysis: A representative lot from an independent laboratory detected NLRC5 in THP-1 cell lysates transfected with a control vector and demonstrated a loss of signal in THP-1 cell lysates transfected with NLRC5 siRNA. The same lot also detected NLRC5 in HeLa cell lysates treated with poly (I:C) (Neerincx, A., et al. (2010). J Biol Chem. 285(34):26223-26232.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated endogenous NLRC5 from cytosolic and nuclear fractions of poly (I:C) treated HeLa cell lysates. Immunoprecipitated NLRC5 was then detected in Western Blotting using a representative lot of this antibody (Neerincx, A., et al. (2012). J Immunol. 188(10):4940-4050.).生物信息 免疫原品种 BSA-conjugated linear peptide corresponding to the C-terminus of human NLRC5. 克隆 3H8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - NOD4
- NLRC5
- NOD27
纯化方法 Affinity UniProt编号 - Q86WI3
产品使用声明 质量保证 Evaluated by Western Blotting in EL4 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NLRC5 in 10 µg of EL4 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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