merck millipore,默克密理博,05-939,Anti-LSD1/BHC110 Antibody

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merck millipore,默克密理博,05-939,Anti-LSD1/BHC110 Antibody
产品名称：Anti-LSD1/BHC110 Antibody
产品型号：05-939
Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, More>>

merck millipore,默克密理博,05-939,Anti-LSD1/BHC110 Antibody

产品介绍

merck millipore,默克密理博,05-939,Anti-LSD1/BHC110 Antibody

Replacement Information
Replacement Information	06-574 is a recommended replacement for CBL770

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ChIP, IHC, WB	M	Ascites	Monoclonal Antibody

描述
产品目录编号	05-939
Replaces	CBL770
品牌系列	Upstate
商名	Upstate
描述	Anti-LSD1/BHC110 Antibody
Alternate Names	lysine-specific histone demethylase 1 BRAF35-HDAC complex protein BHC110 FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit Flavin-containing amine oxidase domain-containing protein 2 amine oxidase (flavin containing) domain 2 lysine (K)-specific demethylase 1 lysine (K)-specific demethylase 1
背景信息	Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

产品信息
格式	Ascites
控制	HeLa cell lysate.
演示	Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.

应用
应用	Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Immunohistochemistry Western Blotting
应用说明	Chromatin Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin. Immunoprecipitation: Recommended. Immunohistochemistry: This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005) Immunofluorescence: Recommended.

生物信息
免疫原品种	GST fusion protein corresponding to residues 23-138 of human LSD1.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes LSD1, Mr 110 kDa.
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001009999
Entrez基因汇总	This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.
基因符号	LSD1 AOF2 KIAA0601 BHC110
纯化方法	Unpurified
UniProt编号	O60341
UniProt汇总	FUNCTION: SwissProt: O60341 # Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri- methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys- 9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. COFACTOR: FAD. SIZE: 852 amino acids; 92903 Da SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitously expressed. DOMAIN: SwissProt: O60341 The SWIRN domain may act as an anchor site for a histone tail. SIMILARITY: Belongs to the flavin monoamine oxidase family. & Contains 1 SWIRM domain.

产品使用声明
质量保证	Routinely evaluated by western blot. Western Blot Analysis: 1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.