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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE936 Replaces MABE512 描述 Anti-ASF-1 Antibody, clone AK-96 Alternate Names - pre-mRNA-splicing factor SF2, P33 subun
- Serine/arginine-rich splicing factor 1
- Alternative-splicing factor 1
- ASF-1
- Splicing factor, arginine/serine-rich 1
背景信息 Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase. 产品信息 格式 Purified 演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-ASF-1 Antibody, clone AK-96 is validated for use in western blotting, IP & ICC for the detection of ASF-1. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of Raji and HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected ASF-1 in BJ fibroblast cells (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 in BJ fibroblast cell lysate (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).生物信息 免疫原品种 Recombinant protein corresponding to human ASF-1. 克隆 AK-96 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001071634
基因符号 - SFRS1
- SRSF1
- ASF
- SF2
- SF2P33
纯化方法 Protein G purified UniProt编号 - Q07955
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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