merck millipore,默克密理愽
  • merck millipore,默克密理博,06-519,Anti-phospho-CREB (Ser133) Antibody

  • merck millipore,默克密理博,06-519,Anti-phospho-CREB (Ser133) Antibody

    产品名称：Anti-phospho-CREB (Ser133) Antibody
    产品型号：06-519
    Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsi More>>

    merck millipore,默克密理博,06-519,Anti-phospho-CREB (Ser133) Antibody

  • 产品介绍
  • merck millipore,默克密理博,06-519,Anti-phospho-CREB (Ser133) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Ht, H, R, MWB, EMSA, IHCRbPurifiedPolyclonal Antibody
    描述
    产品目录编号 06-519
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-phospho-CREB (Ser133) Antibody
    Alternate Names
    • transactivator protein
    • active transcription factor CREB
    • cAMP responsive element binding protein 1
    • cAMP-response element-binding protein-1
    背景信息 CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
    产品信息
    格式 Purified
    控制
    • Forskolin-stimulated WI-38 nuclear extracts.
    演示 Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) 15 mM NaCl, and 0.05% sodium azide with 30% glycerol.
    应用
    应用 Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
    主要应用
    • Western Blotting
    • Electrophoretic Mobility Shift Assay
    • Immunohistochemistry
    应用说明 Immunoprecipitation:
    10 μL of a previous lot immunoprecipitated phosphorylated CREB from 500 μg of cells treated with 50 μM forskolin.

    Immunohistochemistry:
    A 1:1000 dilution of previous lots detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.

    Gel Shift (EMSA):
    This antibody has been shown to gel shift by independent labs.
    生物信息
    免疫原品种 KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
    表位 phospho-Ser133
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
    同种型 IgG
    品种反应性 HamsterHumanRatMouse
    Species Reactivity Note Reacts with human, hamster, mouse and rat.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_134442
    Entrez基因汇总 This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
    基因符号
    • MGC9284
    • CREB1
    • CREB
    修改
    • Phosphorylation
    纯化方法 Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
    UniProt编号
    • P16220
    UniProt汇总 FUNCTION:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
    SUBUNIT: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity.
    SUBCELLULAR: Nucleus.
    PMT:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.
    SIMILARITY: Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain
    产品使用声明
    质量保证 Evaluated by western blot on lysates from mouse NIH/3T3 cells treated with 50 mM forskolin.

    Western Blot Analysis: This antibody detected CREB when phosphorylated on Ser133 at 1:1,000 dilution in forskolin treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.

    Handling Recommendations:
    Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
    Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,06-519,Anti-phospho-CREB (Ser133) Antibody

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