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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3607 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-ASC Antibody Alternate Names - Apoptosis-associated Speck-like Protein containing a CARD
- TMS1
- CARD5
背景信息 Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues. 产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 This Anti-ASC Antibody is validated for use in WB for the detection of ASC. 主要应用 - Western Blotting
应用说明 Western blot: 1:500
HL60 whole cell lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Peptide corresponding to aa 182-195 (RESQSYLVEDLERS) of human ASC. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects a 25 kDa band by western blot, corresponding to ASC/TMS1. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_145182.1
- NM_145183.1
- NM_013258.3
Entrez基因汇总 This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - CARD5
- PYCARD
- TMS1
- MGC10332
- ASC
- hASC
UniProt编号 - Q9ULZ3
UniProt汇总 FUNCTION: SwissProt: Q9ULZ3 # Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
SIZE: 195 amino acids; 21627 Da
SUBUNIT: Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
TISSUE SPECIFICITY: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.DOMAIN:SwissProt: Q9ULZ3 Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
PTM: Phosphorylated.
SIMILARITY: Contains 1 CARD domain. & Contains 1 DAPIN domain.
MISCELLANEOUS: In breast tumorigenesis, methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 4°C for up to 6 months. 包装信息 数量 100 µg
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