merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal

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merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal
产品名称：Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal
产品型号：MABE188
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	IHC, WB, ICC, FC	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABE188
描述	Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal
Alternate Names	DNA replication licensing factor MCM7 minichromosome maintenance complex component 7 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) minichromosome maintenance deficient (S. cerevisiae) 7 minichromosome maintenance deficient 7 homolog of S. cerevisiae Cdc47
背景信息	The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

产品信息
格式	Diluted Culture Supernatant
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry Flow Cytometry
应用说明	Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:40 dilution from a representative lot was used in IP. Flow Cytometry Analysis: 1:40 dilution from a representative lot was used in FC. Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected MCM7 in human lung tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to the C-terminus of human MCM7.
表位	C-terminus
克隆	EP1974Y
宿主	Rabbit
特异性	This antibody recognizes the C-terminus of MCM7.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with Human, Mouse, and Rat.
抗体类型	Monoclonal Antibody
基因符号	P1CDC47 CDC47 MCM2 P1.1-MCM3 CDC47 homolog PNAS146 P85MCM
纯化方法	Unpurified
UniProt编号	P33993
UniProt汇总	FUNCTION: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBUNIT STRUCTURE: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. SUBCELLULAR LOCATION: Nucleus By similarity. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex. SEQUENCE SIMILARITIES: Belongs to the MCM family. Contains 1 MCM domain.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1:10,000 dilution of this antibody detected MCM7 in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.