- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB3574,Anti-Vinculin Antibody, clone VIIF9 (7F9)
产品名称：Anti-Vinculin Antibody, clone VIIF9 (7F9)
产品型号：MAB3574
Anti-Vinculin Antibody, clone VIIF9 (7F9) is an antibody against Vinculin for use in IP, WB, IC, IH(P).
merck millipore,默克密理博,MAB3574,Anti-Vinculin Antibody, clone VIIF9 (7F9)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, H, M, Mk, Po ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3574 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Vinculin Antibody, clone VIIF9 (7F9) 产品信息 格式 Purified 控制 - POSITIVE CONTROL:
Vinculin is present in all cell types.
演示 Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. 应用 应用 Anti-Vinculin Antibody, clone VIIF9 (7F9) is an antibody against Vinculin for use in IP, WB, IC, IH(P). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot. 1:1000; ~117kDa band. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 10% and/or 4-20% (or similar) gradient gel.
Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made). If necessary follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue.
Immunocytochemistry (see application notes below).
Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG. Note that the muscle-specific isoform, meta-vinculin (MW 150 kDa) will co-precipitate.
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR MAB3574
IMMUNOCYTOCHEMISTRY
For cultured cells, use the following method: 1) wash cells twice with buffer "A" (100 mM Pipes (to make it dissolve, titrate with KOH), 0.5mM MgCl2, 0.1 mM EDTA, 0.01M EGTA (to make it dissolve, titrate with KOH), pH 6.9); 2) fix 15 min @RT (3.7% formalin, 0.2% Triton X-100, 2M Glycerol, prepared on buffer A); 3) wash 3 times with buffer "A"; 4) proceed with incubation with primary Abs, etc.生物信息 免疫原品种 Crude smooth muscle extract from normal human adult uterus. 克隆 VIIF9 (7F9) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Vinculin. Cellular localization is Intracellular, associated with cytoskeleton. 同种型 IgG1 品种反应性 RabbitBovineHumanMouseMonkeyPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_014000.2
- NM_003373.3
Entrez基因汇总 Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein. 基因符号 - vinculin
- VCL
- MVCL
- Vinculin
- Metavinculin
- OTTHUMP00000043885
- CMD1W
UniProt编号 - P18206
UniProt汇总 FUNCTION: SwissProt: P18206 # Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.
SIZE: 1134 amino acids; 123799 Da
SUBUNIT: Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Note=Cytoplasmic face of adhesion plaques.
TISSUE SPECIFICITY: Metavinculin is muscle-specific.
PTM: Phosphorylated on serines, threonines and tyrosines and acylated by myristic acid and/or palmitic acid.
DISEASE:SwissProt: P18206 # Defects in VCL are a cause of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM).
SIMILARITY: SwissProt: P18206 ## Belongs to the vinculin/alpha-catenin family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,HTS101RTA,Ready-to-Assay™ Mu Opioid Receptor Frozen Cells
下一件merck millipore产品：merck millipore,默克密理博,ABC322,Anti-UQCRFS1 Antibody
相关产品
merck millipore,默克密理博,20-190,TBS, 20X
merck millipore,默克密理博,05-23-2540,Amylin, Human - CAS 122384-88-7 - Calbiochem
merck millipore,默克密理博,ABN349,Anti-BRAL1 Antibody
merck millipore,默克密理博,SF-1500,CXCL12; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,HTS101RTA,Ready-to-Assay™ Mu Opioid Receptor Frozen Cells
merck millipore,默克密理博,ABC322,Anti-UQCRFS1 Antibody
merck millipore,默克密理博,04-135,Anti-Brachyury Antibody, clone 3E4.2
merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal
merck millipore,默克密理博,MABE188,Anti-MCM7 Antibody, clone EP1974Y, rabbit monoclonal