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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MABD117 描述 Anti-GATA Antibody3, clone 7B5 Alternate Names - GATA-binding factor 3
- Trans-acting T-cell-specific transcription factor GATA-3
背景信息 GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear. 产品信息 格式 Ascites 控制 - GATA3-hIgGFc transfected HEK293 cell lysate
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recombinant fragment of human GATA3 expressed in E. Coli. 克隆 7B5 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - GATA3
纯化方法 Unpurified UniProt编号 - P23771
产品使用声明 质量保证 Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.
Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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