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merck millipore,默克密理博,MABC675,Anti-HSPA5 Antibody, clone 4E3
产品名称：Anti-HSPA5 Antibody, clone 4E3
产品型号：MABC675
Anti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC.
merck millipore,默克密理博,MABC675,Anti-HSPA5 Antibody, clone 4E3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R IHC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MABC675 描述 Anti-HSPA5 Antibody, clone 4E3 Alternate Names - BiP
- 78 kDa glucose-regulated protein
- GRP-78
- Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
- Heat shock 70 kDa protein 5
- Immunoglobulin heavy chain-binding protein
背景信息 The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER. EMD-Millipore’s Anti-HSPA5 protein mouse monoclonal antibody, clone 4E3, has been tested by western blot in human HeLa and Jurkat cell lysates as well as mouse NIH3T3 cell lysates. The clone has also been successfully tested in immunohistochemistry in human, paraffin-embedded, thyroid tissues. 产品信息 格式 Ascites 控制 - HeLa cell lysate
演示 Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Anti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HSPA5 in 10 µg of NIH/3T3, Jurkat, and A431 cell lysates.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected HSPA5 in rat cerebellum, rat pons/midbrain, rat hippocampus, and human thyroid tissues.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recominant fragment of human HSPA5 expressed in E. coli. 克隆 4E3 宿主 Mouse 同种型 IgG1 品种反应性 MouseHumanRat 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005338
基因符号 - GRP78
- HSPA5
纯化方法 Unpurified UniProt编号 - P11021
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected HSPA5 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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