merck millipore,默克密理博,MABS176,Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal

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merck millipore,默克密理博,MABS176,Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal
产品名称：Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal
产品型号：MABS176
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Adiponectin Antibody, clone EPR3217, Rabbi More>>

merck millipore,默克密理博,MABS176,Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABS176,Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABS176
描述	Anti-Adiponectin Antibody, clone EPR3217, rabbit monoclonal
Alternate Names	Adipocyte, C1q and collagen domain-containing protein adiponectin, C1Q and collagen domain containing Adipocyte complement-related 30 kDa protein Adipose most abundant gene transcript 1 protein 30 kDa adipocyte complement-related protein adipocyte, C1Q and collagen domain containing gelatin-binding protein 28 adipose specific collagen-like factor Gelatin-binding protein
背景信息	Adiponectin is a multimeric adipocyte-specific secretory protein that regulates energy homeostasis, lipid metabolism, and insulin sensitivity with direct anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin predominantly inhibits proliferation of myelomonocytic lineage cells and is an important negative regulator in hematopoiesis and immune systems (1, 2). Adiponectin is reported to possibly function as an endogenous factor involved in lipid catabolism and storage or whole body metabolism (3). It is decreased in obesity and has been demonstrated to inhibit tumor necrosis factor-alpha (TNF-alpha)-induced expression of endothelial adhesion molecules. Adiponectin modulates the inflammatory response of endothelial cells, possibly through cross talk between cAMP-PKA and NF-kappaB signaling pathways (4). Adiponectin forms trimers, hexamers and 12- to 18-mers. The trimers are low molecular weight complexes (LMW). Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes (MMW)) and higher molecular weight (HMW) complexes (5).

产品信息
格式	Diluted Culture Supernatant
控制	Human fetal fat and human plasma lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Adiponectin Antibody, clone EPR3217, Rabbit is validated for use in WB, IC for the detection of Adiponectin.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Western Blot Analysis: 1:2,000 - 5,000 dilution of this antibody detected Adiponectin in human plasma lysates treated with (A) Beta-ME and heat, (B) heat only, (C) Beta-ME only, and (D) no treatment.

生物信息
免疫原品种	Synthetic peptide corresponding to human Adiponectin.
克隆	EPR3217
宿主	Rabbit
同种型	IgG
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q15848
基因符号	apM-1 GBP28 ACRP3 APM1 adiponectin ACDC ADIPQTL1 AdipoQ apM1 OTTHUMP00000210047 ADPN APM-1
纯化方法	Unpurified
UniProt编号	Q15848
UniProt汇总	FUNCTION: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. SUBUNIT STRUCTURE: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex) By similarity. SUBCELLULAR LOCATIOIN: Secreted. TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma. DOMAIN: The C1q domain is commonly called the globular domain. PTM: Hydroxylated Lys-33 was not identified in Ref.11, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes By similarity. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. Polymorphism Genetic variations in ADIPOQ influence the variance in adiponectin serum levels and define the adiponectin serum levels quantitative trait locus 1 (ADIPQTL1) [MIM:612556]. INVOLEMENT IN DISEASE: Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin. Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. PHARMACEUTICAL USE: Adiponectin might be used in the treatment of diabetes type 2 and insulin resistance. MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW-complex secretion By similarity. In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls. SEQUENCE SIMILARITIES: Contains 1 C1q domain. Contains 1 collagen-like domain.

产品使用声明
质量保证	Evaluated by Western Blot in human fetal fat and human plasma lysates. Western Blot Analysis: 1:2,000 - 5,000 dilution of this antibody detected Adiponectin in human fetal fat and human plasma lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.