- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABC667,Anti-GPC3 Antibody, clone 9C2
产品名称：Anti-GPC3 Antibody, clone 9C2
产品型号：MABC667
Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
merck millipore,默克密理博,MABC667,Anti-GPC3 Antibody, clone 9C2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H FC, WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABC667 描述 Anti-GPC3 Antibody, clone 9C2 Alternate Names - Intestinal protein OCI-5
- SGB
- DGSX
- MXR7
- SDYS
- SGBS
- OCI-5
- SGBS1
- GTR2-2
- Glypican-3
背景信息 Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA. 产品信息 格式 Ascites 控制 - Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
演示 Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer. 应用 应用 Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human GPC3 expressed in E. Coli. 克隆 9C2 宿主 Mouse 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody 基因符号 - OCI5
- GPC3
纯化方法 Protein G purified UniProt编号 - P51654
产品使用声明 质量保证 Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,ABE416,Anti-SHMT1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,MABE525,Anti-RRM1, clone EPR8482, Rabbit Monoclonal Antibody
相关产品
merck millipore,默克密理博,03-200,RIPAb+ SMN - RIP Validated Antibody and Primer Set
merck millipore,默克密理博,S7117,ApopTag Plastic Coverslip
merck millipore,默克密理博,343021,Flt-3 Inhibitor II - CAS 896138-40-2 - Calbiochem
merck millipore,默克密理博,1690-OP,OmniPur Acrylamide Bis-acrylamide, 29:1, 40% Solution - Calbiochem
merck millipore,默克密理博,ABE416,Anti-SHMT1 Antibody
merck millipore,默克密理博,MABE525,Anti-RRM1, clone EPR8482, Rabbit Monoclonal Antibody
merck millipore,默克密理博,06-578,Anti-PI4-Kinase β Antibody
merck millipore,默克密理博,SF-759,TNFAIP3; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,SF-2582,MKNK2; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,04-227,Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal