merck millipore,默克密理博,04-227,Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal

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merck millipore,默克密理博,04-227,Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
产品名称：Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
产品型号：04-227
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Estrogen Receptor α Antibody, clone E115 i More>>

merck millipore,默克密理博,04-227,Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-227,Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-227
品牌系列	Upstate
商名	Upstate
描述	Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
Alternate Names	steroid hormone receptor Estradiol receptor Nuclear receptor subfamily 3 group A member 1 estrogen receptor 1 estrogen receptor alpha oestrogen receptor
背景信息	Estrogen Receptor α (ER alpha) is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

产品信息
格式	Culture Supernatant
控制	MCF-7 cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Estrogen Receptor α Antibody, clone E115 is validated for use in WB, IC, IH(P) for the detection of Estrogen Receptor α.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry: Immunocytochemistry: 1:100 dilution of a previous lot was used on immunocytochemistry.

生物信息
免疫原品种	Synthetic peptide directed against human Estrogen Receptor α.
克隆	E115
宿主	Rabbit
特异性	This antibody recognizes Estrogen Receptor α.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_000116.2
Entrez基因汇总	This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq].
基因符号	OTTHUMP00000017719 ER ER-alpha ESR ESRA Era NR3A1 DKFZp686N23123 OTTHUMP00000017718
纯化方法	Unpurified
UniProt编号	P03372
UniProt汇总	FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.

产品使用声明
质量保证	Routinely evaluated by western blot on MCF-7 cell lysate. Western Blot Analysis: A 1:250-1:500 dilution of this lot was used to detect Estrogen Receptor  in MCF-7 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.