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Replacement Information Replacement Information 07-1217 is a recommended replacement for AB4083 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1217 Replaces AB4083 品牌系列 Upstate
商名 - Upstate
描述 Anti-PP1β Antibody Alternate Names - Protein phosphatase 1-delta
- Serine/threonine-protein phosphatase PP1-beta catalytic subunit
- Protein phosphatase 1, catalytic subunit, beta
- Protein phosphatase 1, catalytic subunit, beta isoform
- Protein phosphatase 1-beta
- beta isoform
- Protein phosphatase 1-beta
背景信息 Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR. 产品信息 格式 Purified 控制 - HeLa Cell Lysate
演示 Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide. 应用 应用 This Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β. 主要应用 - Western Blotting
应用说明 Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB.
生物信息 免疫原品种 The immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327). 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms. 同种型 IgG 品种反应性 RatBovineHumanMouse Species Reactivity Note Bovine,Human, Mouse, Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_206877
Entrez基因汇总 The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - EC 3.1.3.16
- PPP1CB
- PP-1B
- PPP1CD
- MGC3672
纯化方法 Purified UniProt编号 - P62140
UniProt汇总 FUNCTION: SwissProt: P62140 # Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 327 amino acids; 37187 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen (By similarity). Interacts with PPP1R7.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: SwissProt: P62140 ## Belongs to the PPP phosphatase family. PP-1 subfamily.产品使用声明 质量保证 Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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