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重要规格表
可用的分析物 品种反应性 主要应用 检测方法 GFAP H, M, R ELISA Chromogenic 描述 产品目录编号 NS830 品牌系列 Chemicon®
商名 - Chemicon
描述 GFAP ELISA Kit 概述 Background
Glial Fibrillary Acidic Protein (GFAP) is a class-III intermediate filament uniquely and ubiquitously expressed as part of the structural network in astrocytic glial cells. This specific expression makes GFAP ideal as a broad astrocytic marker in vertebrates. Astrocytes play a variety of key roles in supporting, guiding, nurturing, and signaling neuronal architecture and activity in a highly interdependent manner. Current understanding of CNS structure suggests that there are at least as many glial cells as the estimated 100 billion neurons, but perhaps upwards of 100 times more. Thus,
the health of both neurons and astrocytes is often affected in disease and trauma. GFAP is proving to be a useful biomarker in the detection of neural degeneration. Although defects in GFAP have been reported to cause certain rare conditions, such as Alexander’s disease (Brenner et al., 2001), the observed interconnected physiology of neuronal and glial cells has enabled the use of GFAP as a neurodegeneration detection gauge. This relationship is being exploited by researchers and clinicians measuring a variety of neurological issues from cancer to trauma. GFAP has been used as a biomarker in the early detection of chemically induced cancer (Capo et al., 1997) and classical glioblastomas (Jung et al., 2007). Elevated levels of GFAP have been reported in traumatic brain injury (TBI) and appear to directly correlate with the severity of injury and brain tissue oxygenation in combat casualty care research (CCR, 2008) as well as civilian accidents (Mondello et al., 2010). GFAP has also been identified as a good biomarker in studies of toxin induced acute brain injury (Liao et al., 2008), in differentiation of hemorrhagic vs. ischemic strokes (Foerch et al., 2006), as well as in cardiac arrest (Kaneko et al., 2009). Millipore now offers an ELISA assay for sensitive measurement of GFAP protein in tissue homogenates and biological fluids (serum, plasma, CSF, and serum free media) from human, mouse, and rat.
Principals of Procedure
This assay is a Sandwich ELISA based sequentially on: 1) capture of soluble GFAP protein to the wells of a microtiter plate coated by a pre-titered amount of anti-GFAP monoclonal antibody; 2) removal of unbound material by washing; 3) binding of biotinylated anti-GFAP monoclonal antibody to
the previously captured GFAP protein; 4) removal of unbound material by washing; 5) binding of streptavidin conjugated horseradish peroxidase to the biotinylated antibodies; 6) removal of excess or unbound enzyme conjugates by washing; and 7) quantification of immobilized antibody-enzyme
complexes by monitoring horseradish peroxidase activity in the presence of the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). Enzyme activity is measured spectrophotometrically by recording absorbance readings at 450 nm. Increases in absorbency are directly proportional to the amount of
GFAP protein captured from the sample. Therefore the protein concentration of unknowns can be derived by interpolation using a standard curve generated in the same assay with reference standards of known concentrations of GFAP.
Alternate Names - Glial Fibrillary Acidic Protein ELISA
- GFAP
产品信息 部件 - Plate Sealers (CS200443): 10 Sheets
- 96-Well Anti-GFAP Coated Plate (CS206352): 1 Plate
- 1X Assay Buffer (CS206357): 40 mL
- 10X Wash Buffer (CS206360): 50 mL
- GFAP Protein Standard (CS206361): 1 Vial
- GFAP Quality Control #1 (CS206351): 1 Vial
- GFAP Quality Control #2 (CS206475): 1 Vial
- Biotinylated Anti-GFAP Detection Antibody (CS206356): 12 mL
- Enzyme Solution (CS206358): 12 mL
- TMB Solution (CS202682): 10 mL
- Stop Solution (60193): 12 mL
检测方法 Chromogenic 应用 主要应用 - ELISA
生物信息 品种反应性 HumanMouseRat 可用的分析物 - GFAP
Entrez基因编号 - NM_002055.2
Entrez基因汇总 This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. 基因符号 - GFAP
UniProt编号 - P14136
UniProt汇总 FUNCTION: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with intermediate filaments.
TISSUE SPECIFICITY: Expressed in cells lacking fibronectin.
PTM: Phosphorylated by PKN1.
SIMILARITY: Belongs to the intermediate filament family.理化信息 灵敏度 - 1.5 ng/mL
标准曲线范围 - 1.5 to 100 ng/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Storage: The GFAP ELISA kit is shipped on ice and should be stored at 2-8°C upon arrival.
Stability: Kit components are stable for a minimum of 6 months from the date of receipt if stored and
handled as described above.
包装信息 数量 1 kit 包装 96 wells
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