- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MABC60 描述 Anti-KiSS-1 Antibody, clone 8H4.1 Alternate Names - Kisspeptin-10
- Metastasis-suppressor KiSS-1
- Kisspeptin-1
- Metastin
- Kisspeptin-542
- Kisspeptin-143
- Kisspeptin-134
背景信息 Metastasis-suppressor KiSS-1 (KiSS-1; or Kisspeptin-1) is one of several endogenous ligands for the orphan G-protein coupled receptor GPR54. KiSS-1 functions as a suppressor of metastasis in various types of cancers, by inhibiting migration and proliferation in affected cells. This effect may be produced by the activation of multiple signaling pathways: Ligand-bound GPR54 activates IP3 and PKC signaling via a PLC pathway; and ERK 1 and ERK 2 by a cPLA2, which may lead to increased calcium signaling and gene transcription, respectively. More recently, however, KiSS-1/GPR54 signaling has been implicated in puberty and development. KiSS-1/GPR54 may regulate signaling of GnRH neurons during puberty and may influence the release of FH and LSH hormones. KISS-1 is highly expressed in the CNS, testis, pancreas, intestine, ovary, and placenta. 产品信息 格式 Purified 控制 - Human placenta tissue
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-KiSS-1 Antibody, clone 8H4.1 is an antibody against KiSS-1 for use in IHC, Western Blotting. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected KiSS-1 in human pancreas and rat testis tissues. 生物信息 免疫原品种 Recombinant protein corresponding to human KiSS-1. 克隆 8H4.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react in Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002247
Entrez基因汇总 This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Jul 2008]. 基因符号 - PP5098
- KISS1
纯化方法 Protein G purified UniProt编号 - Q15726
UniProt汇总 FUNCTION: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast.
INDUCTION: Down-regulated during the progression of melanoma in vivo. Diminishes MMP9 expression by effecting reduced NF-kappa-B binding to the promoter.
PTM: Processed by MMP2 and MMP9.
SEQUENCE SIMILARITIES: Belongs to the KISS1 family.
SEQUENCE CAUTION: The sequence AAC79512.1 differs from that shown. Reason: Frameshift at position 138.
The sequence AAM78592.1 differs from that shown. Reason: Frameshift at position 138.产品使用声明 质量保证 Evaluated by Immunohistochemistry in human placenta tissue.
Immunohistochemistry Analysis: 1:1,000 dilution of this antibody detected KiSS-1 in human placenta tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,04-1486,Anti-33D1 (CD8- Mouse DC marker) Antibody, clone 33D1
下一件merck millipore产品：merck millipore,默克密理博,07-276,Anti-PDGFRα Antibody
相关产品
merck millipore,默克密理博,SLMP025SS,Millex-GP Med，0.22 µm，改良聚醚砜，25 mm，环氧乙烷灭菌
merck millipore,默克密理博,361569,GSK-3beta Inhibitor XXVI - Calbiochem
merck millipore,默克密理博,AB9626,Anti-Ephrin B phosphoTyr317 Antibody
merck millipore,默克密理博,04-1486,Anti-33D1 (CD8- Mouse DC marker) Antibody, clone 33D1
merck millipore,默克密理博,07-276,Anti-PDGFRα Antibody
merck millipore,默克密理博,218759,Caspase-8 Inhibitor II - Calbiochem
merck millipore,默克密理博,412400,IQ-1 - CAS 331001-62-8 - Calbiochem
merck millipore,默克密理博,616395,Transferrin, Apo-, Human Plasma
