merck millipore,默克密理博,MABC199,Anti-BRCA1 Antibody, clone MS110

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABC199
描述	Anti-BRCA1 Antibody, clone MS110
Alternate Names	RING finger protein 53 Breast cancer type 1 susceptibility protein
背景信息	Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.

产品信息
格式	Purified
控制	HeLa nuclear extract
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells. Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).

生物信息
免疫原品种	Recombinant protein corresponding to the N-terminus of human BRCA1.
表位	N-terminus
克隆	MS110
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes the N-terminus of BRCA1.
同种型	IgG1κ
品种反应性	Human
Species Reactivity Note	Demonstrated to react in Human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_009225
基因符号	RNF53 BRCA1
纯化方法	Protein G purified
UniProt编号	P38398

产品使用声明
质量保证	Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MABC199,Anti-BRCA1 Antibody, clone MS110

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech