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merck millipore,默克密理博,MAB5448,Anti-Neurofilament NF-H Antibody, clone TA51
产品名称：Anti-Neurofilament NF-H Antibody, clone TA51
产品型号：MAB5448
Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
merck millipore,默克密理博,MAB5448,Anti-Neurofilament NF-H Antibody, clone TA51
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ht, Mk IHC, ELISA, WB R Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB5448 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament NF-H Antibody, clone TA51 Alternate Names - NF-H
产品信息 格式 Culture Supernatant 演示 Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. 应用 应用 Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g
for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}
Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.
Optimal working dilutions must be determined by end user.生物信息 表位 carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH 克隆 TA51 宿主 Rat 特异性 Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}. 同种型 IgG 品种反应性 RatHumanHamsterMonkey 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_021076.2
基因符号 - KIAA0845
- NEFH
- NFH
- NF-H
修改 - Phosphorylation
UniProt编号 - P12036
UniProt汇总 FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 1 mL
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