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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5316 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Rhodopsin Antibody, clone RET-P1 Alternate Names - Opsin 2
产品信息 格式 Purified 控制 - POSITIVE CONTROL:
IMR-5 cells, brain or retina.
演示 Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide. 应用 应用 Detect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoblotting: 1 μg/mL
Immunocytochemistry
Immunohistochemistry (frozen and formalin/paraffin): 1-2 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Membrane preparation from adult rat retina. 克隆 RET-P1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.
CELLULAR LOCALIZATION:
Cytoplasmic同种型 IgG1 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000539.2
Entrez基因汇总 Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. 基因符号 - MGC138309
- RHO
- rhodopsin
- Opsin-2
- OPN2
- Rhodopsin
- RP4
- MGC138311
UniProt编号 - P08100
UniProt汇总 FUNCTION: SwissProt: P08100 # Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
SIZE: 348 amino acids; 38893 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE: SwissProt: P08100 # Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4) [MIM:180380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant. & Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. & Defects in RHO are a cause of autosomal dominant congenital stationary night blindness 1 (CSNBAD1) [MIM:610445]; also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a nonprogressive retinal disorder characterized by impaired night vision and ocular symptoms such as myopia, hyperopia, nystagmus and reduced visual acuity.
SIMILARITY: SwissProt: P08100 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months. 包装信息 数量 500 µL
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