merck millipore,默克密理博,SCM133,PluriSTEM™ Dispase-II Solution

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merck millipore,默克密理博,SCM133,PluriSTEM™ Dispase-II Solution
产品名称：PluriSTEM™ Dispase-II Solution
产品型号：SCM133

merck millipore,默克密理博,SCM133,PluriSTEM™ Dispase-II Solution

产品介绍

merck millipore,默克密理博,SCM133,PluriSTEM™ Dispase-II Solution

重要规格表

主要应用
CULT

描述
产品目录编号	SCM133
商名	PluriSTEM
描述	PluriSTEM™ Dispase-II Solution
Alternate Names	Dispase 2 Dispase
背景信息	Proteolytic enzymes such as trypsin, collagenase and pronase are commonly used for dispersing tissues and cells. These enzymes, however, often injure the cells, are unstable during incubation, can be heterogeneous and also a source of mycoplasma contamination. Dispase-II is used for the preparation of cells from a wide variety of different tissues and organs. Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. PluriSTEM Dispase-II has been validated to work alongside PluriSTEM Human ES/iPS Medium (SCM130) for the culture and passage of human embryonic and induced pluripotent stem cells. Dispase-II enzyme is produced in Bacillus polymyxa.

产品信息
配方
演示	Product is presented in sterile DMEMF12 at 1 mg/ml. Product is filtered through a 0.2 micron filter before freezing.

应用
主要应用	Cell Culture
应用说明	Optimal passaging technique must be determined by end user and varies depending on cell type and culture conditions. 1. Coat 6-well plates with 1:20 Matrigel coating (1.5 ml per well). Incubate at 2-8ºC overnight or coat at RT for at least 30 minutes to 1 hour before use. 2. Remove matrigel coating. Add 2 mL of Complete Media per well. 3. Remove areas of differentiation within culture. 4. Aspirate media. 5. Wash once with 2 mL PBS. 6. Add 1.5 mL Dispase II per well. Incubate for 37ºC for 7 minutes. 7. Wash 2X with 2 mL PBS, w/o Mg and Ca. 8. Add 2 mL Complete Media to each well and use cell scraper to detach colonies. 9. Collect scrapped cells in 15 ml conical tube. 10. Spin 800 rpm for 5 minutes 11. Resuspend in appropriate volume of complete media. Typical splitting ratio is 1:5 – 1:6 depending upon cell density. Cells should be ready for passaging in 5-6 days time using PluriSTEM Human ES/iPS Medium.

应用

主要应用

Cell Culture

应用说明

Optimal passaging technique must be determined by end user and varies depending on cell type and culture conditions.

1. Coat 6-well plates with 1:20 Matrigel coating (1.5 ml per well). Incubate at 2-8ºC overnight or coat at RT for at least 30 minutes to 1 hour before use.
2. Remove matrigel coating. Add 2 mL of Complete Media per well.
3. Remove areas of differentiation within culture.
4. Aspirate media.
5. Wash once with 2 mL PBS.
6. Add 1.5 mL Dispase II per well. Incubate for 37ºC for 7 minutes.
7. Wash 2X with 2 mL PBS, w/o Mg and Ca.
8. Add 2 mL Complete Media to each well and use cell scraper to detach colonies.
9. Collect scrapped cells in 15 ml conical tube.
10. Spin 800 rpm for 5 minutes
11. Resuspend in appropriate volume of complete media. Typical splitting ratio is 1:5 – 1:6 depending upon cell density. Cells should be ready for passaging in 5-6 days time using PluriSTEM Human ES/iPS Medium.

生物信息
浓缩	Product is presented in sterile DMEMF12 at 1 mg/ml.

产品使用声明
质量保证	Solution is red with no particulates present. Solution is sterile and suitable for cell culture applications.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -20°C for up to 4 months from date of receipt. Once thawed, aliquot in smaller working volumes and store at -20ºC. Avoid multiple freeze thaw cycles to maintain proper enzymatic activity.