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Replacement Information 描述 概述 Protein glycosylation influences crucial biological and pharmaceutically relevant events. Glycoanalysis studies of antibodies, receptors and other glycosylated proteins require highly sensitive, selective, efficient and reproducible methods for sample preparation and mass spectrometry (MS). Effective analysis requires glycopeptide enrichment to compensate for low abundance glycopeptides in complex mixtures and, subsequently, to enable glycan and attached peptide analysis by MS. The ProteoExtract® Glycopeptide Enrichment Kit is designed to capture high-mannose, hybrid, and complex type glycosylated peptides from complex protein mixtures. The kit uses a proprietary ZIC® Glycocapture Resin. In combination with the included optimized buffers, the ZIC Glycocapture Resin is highly selective for glycosylated peptides in the presence of abundant non-glycosylated peptides. The protocol is optimized to allow efficient removal of non-glycosylated peptides with an almost complete glycopeptide recovery (as quantitatively assessed by N-Glycan mapping). It allows both site-specific determination of the glycan structure and parallel analysis of the attached peptides. Glycopeptide enrichment is non-specific and nonselective, without preference for particular glycan structures and without loss of distinct glycans. The enriched glycopeptide fraction is directly compatible with downstream analysis by MALDI and ESI-MS. One ProteoExtract® Glycopeptide Enrichment Kit provides sufficient reagents for 50 enrichment reactions. 10 µl sample with total protein concentration 2-4 mg/ml. 产品目录编号 72103 品牌系列 Novagen®
应用数据 
The Glycopeptide Enrichment Kit allows for selective and sensitive glycopeptide enrichment with low background from complex mixtures. A complex peptide mixture derived from a tryptic digest of bovine fetuin, α1 acid glycoprotein, RNaseB, histone, BSA, and human IgG was processed using the ProteoExtract® Glycopeptide Enrichment Kit as outlined in the User Protocol TB527. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mod. A. Sum spectra of unprocessed sample. B. Sum spectra of PNGaseF digested glycopeptides recovered using the ProteoExtract Glycopeptide Enrichment Kit.参考 产品信息 应用 生物信息 理化信息 尺寸 材料信息 毒性学信息 GHS安全信息 安全信息 产品使用声明 储存和货运信息 货运代码 Ambient or Blue Ice
毒性 Flammable
储存 +2°C to +8°C
无需冷冻 No
包装信息 运输信息 补充信息 规格
上一件merck millipore产品：merck millipore,默克密理博,AB1975,Anti-Enkephalin, Met Antibody
下一件merck millipore产品：merck millipore,默克密理博,MAB5316,Anti-Rhodopsin Antibody, clone RET-P1
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