- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB4603,Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
产品名称：Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
产品型号：MAB4603
Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
merck millipore,默克密理博,MAB4603,Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB4603 Replaces 04-440; 04-1088 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 Alternate Names - CPP32
产品信息 格式 Purified 演示 Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide. 应用 应用 Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P). 主要应用 - Western Blotting
应用说明 Western blot: 1 μg/ml for 2 hours at RT.
Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant full-length human caspase-3 protein 表位 large subunit & proform 克隆 3CSP03 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis. 同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_032991.2
- NM_004346.3
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. 基因符号 - Caspase-3 subunit p12].
- CASP3
- apopain
- CASP-3
- procaspase3
- CPP32B
- SCA-1
- CPP-32
- Apopain
- CPP32
- Yama
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17
UniProt编号 - P42574
UniProt汇总 FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,MABS193,Anti-v-Src Antibody, clone 327
下一件merck millipore产品：merck millipore,默克密理博,AG678,Connexin 43, control peptide for MAB3067
相关产品
merck millipore,默克密理博,12-536,PKCtide
merck millipore,默克密理博,AB2302,Anti-GAPDH Antibody
merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
merck millipore,默克密理博,MABS193,Anti-v-Src Antibody, clone 327
merck millipore,默克密理博,AG678,Connexin 43, control peptide for MAB3067
merck millipore,默克密理博,14-429M,MEK1 Protein, active, 250 µg
merck millipore,默克密理博,MABT140,Anti-Claudin-4 Antibody, clone 10B4.3
merck millipore,默克密理博,PIMP30R48,Millicell 悬挂式细胞培养皿, PET 5.0 µm
merck millipore,默克密理博,OP64,Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)