merck millipore,默克密理博,OP64,Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

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merck millipore,默克密理博,OP64,Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)
产品名称：Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)
产品型号：OP64
This Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) is validated for use in FC, Immunoblotting, IF, IP, Paraffin Sections for the detection of p21WAF1 (Ab-1).

merck millipore,默克密理博,OP64,Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

产品介绍

merck millipore,默克密理博,OP64,Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

重要规格表

宿主
M

描述
概述	Recognizes the ~21 kDa p21^WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
产品目录编号	OP64
品牌系列	Calbiochem®
同义词	Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
应用数据	Detection of p21 WAF1 by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-p21^WAF1 (Ab-1) Mouse mAb (EA10) (Cat. No. OP64) (3 µg/ml). Secondary antibody: Anti-Mouse IgG (Goat) Peroxidase Conjugate. Detection: chemiluminescence.

参考
参考	Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493. Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889. Deng, C., et al. 1995. Cell 82, 675. El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301. El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169. Li, R., et al. 1994. Nature 371, 534. Michieli, P., et al. 1994. Cancer Res. 54, 3391. Noda, A., et al.1994. Exp. Cell Res. 211, 90. El-Deiry, W.S., et al.1993. Cell 75, 817. Gu, Y., et al. 1993. Nature 366, 707. Harper, J.W., et al.1993. Cell 75, 805. Xiong, Y., et al.1993. Genes Devel. 7, 1572. Xiong, Y., et al.1993. Nature 366, 701. Xiong, Y., et al.1992. Cell 71, 505.

参考

Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
Deng, C., et al. 1995. Cell 82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
Li, R., et al. 1994. Nature 371, 534.
Michieli, P., et al. 1994. Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res. 211, 90.
El-Deiry, W.S., et al.1993. Cell 75, 817.
Gu, Y., et al. 1993. Nature 366, 707.
Harper, J.W., et al.1993. Cell 75, 805.
Xiong, Y., et al.1993. Genes Devel. 7, 1572.
Xiong, Y., et al.1993. Nature 366, 701.
Xiong, Y., et al.1992. Cell 71, 505.

产品信息
形式	Liquid
配方	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
负控制	Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
正控制	Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
防腐剂	≤0.1% sodium azide

应用
应用参考	Epitope Identification Patrick O'Connor (NCI, personal communication). Original Clone, Paraffin Sections, Frozen Sections El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Paraffin Sections, Antigen Retrieval Mouriaux, Frédéric, et al. 2000. Invest Ophthamol. Vis. Sci. 41, 2837. Flow Cytometry Carter, A., et al. 2004. Brit. J. Haematol. 127, 425.
主要应用	Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections

生物信息
免疫原	full-length, recombinant, human p21^WAF1
免疫原品种	Human
表位	within amino acids 58-77 of human p21^WAF1
克隆	EA10
宿主	Mouse
同种型	IgG₁
品种	human, not mouse, not rat
浓缩标签	Please refer to vial label for lot-specific concentration