- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, M, R, Eq WB Ch Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2302 描述 Anti-GAPDH Antibody Alternate Names - glyceraldehyde-3-phosphate dehydrogenase
- glyceraldehyde 3-phosphate dehydrogenase
- aging-associated gene 9 protein
背景信息 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). 产品信息 格式 Affinity Purified 控制 - HEK293 cell lysate
演示 Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH. 表位 WARS interacting region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 This antibody recognizes GAPDH. 品种反应性 CanineHumanMouseRatHorse Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with equine and canine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002037
Entrez基因汇总 The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]. 基因符号 - G3PD
- GAPDH
- G3PDH
- GAPD
纯化方法 Affinity Purfied UniProt编号 - P04406
UniProt汇总 FUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
SIZE: 335 amino acids; 36053 Da
SUBUNIT: Homotetramer. Binds PRKCI.
SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,12-536,PKCtide
下一件merck millipore产品：merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
相关产品
merck millipore,默克密理博,SF-138,GAPDH; Mouse, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,07-641,Anti-MEK1 Antibody
merck millipore,默克密理博,14-335,PRAK Protein, unactive, 50 µg
merck millipore,默克密理博,12-536,PKCtide
merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
merck millipore,默克密理博,MABS193,Anti-v-Src Antibody, clone 327
merck millipore,默克密理博,MAB4603,Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
merck millipore,默克密理博,AG678,Connexin 43, control peptide for MAB3067