merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

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merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
产品名称：Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
产品型号：04-403
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibod More>>

merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-403,Anti-PI3 Kinase Antibody, p85α rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, FC, ICC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-403
品牌系列	Upstate
商名	Upstate
描述	Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
Alternate Names	phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85<br />alpha). PI3-kinase p85 subunit alpha PtdIns-3-kinase p85-alpha phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85<br />alpha) phosphatidylinositol 3-kinase, regulatory, 1 phosphatidylinositol 3-kinase-associated p-85 alpha phosphoinositide-3-kinase, regulatory subunit 1 (alpha) phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha) phosphatidylinositol 3-kinase-associated p-85 alpha phosphoinositide-3-kinase regulatory subunit 1 (alpha) regulatory subunit 1 (p85 alpha)
背景信息	PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

产品信息
格式	Purified
控制	Lysates from A431, MCF-7 or Raji cells
演示	Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p85α Rabbit validated for use in FC, IC, IF, IH(P), IP & WB.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry
应用说明	Immunoprecipitation: A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3K, p85α using protein G:agarose beads. Flow Cytometry: A 1:50 dilution of a previous lot of this antibody stained cells for intracellular staining for flow cytometry analysis.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p85α subunit.
表位	C-terminus
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes the C-terminal domain of PI3 Kinase, p85α subunit.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse and rat. Reactivity with other species has not been confirmed.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_181504.2
Entrez基因汇总	Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
基因符号	p85-ALPHA PIK3R1 GRB1 PI3K
纯化方法	Protein A purfied
UniProt编号	P27986
UniProt汇总	FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. SIZE: 724 amino acids; 83598 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1. TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance. SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on A431 lysate. Western Blot Analysis: A 1:2,000 dilution of the lot detected endogenous PI3K, p85α in lysates from A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.