- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB4334,Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
产品名称：Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
产品型号：MAB4334
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).
merck millipore,默克密理博,MAB4334,Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Pm, H IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4334 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 Alternate Names - Pgp
- P-glycoprotein
- CD243
- p170
背景信息 UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905). 产品信息 格式 Purified 演示 The immunoglobulin was purified by protein A Sepharose™ chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative. 应用 应用 Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P). 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.
Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.
Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.
Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.
IHC on frozen and paraffin sections: 1 - 10 μg/mL.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine. 表位 conformational extracellular epitope 克隆 UIC2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243) 同种型 IgG2aκ 品种反应性 PrimateHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000927.3
Entrez基因汇总 The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. 基因符号 - EC 3.6.3.44
- ABCB1
- PGY1
- MGC163296
- CD243
- GP170
- P-gp
- MDR1
- CLCS
- ABC20
非反应性品种 - Rat
- Mouse
UniProt编号 - P08183
UniProt汇总 FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up 12 months from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,506123,Anti-p38 MAP Kinase (341-360) Rabbit pAb
下一件merck millipore产品：merck millipore,默克密理博,09-126,Anti-Tribbles-1 Antibody
相关产品
merck millipore,默克密理博,ABN34,Anti-PDE11A Antibody
merck millipore,默克密理博,SF-158,PTPN11; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MABN740,Anti-GABA-B receptor 2 Antibody, clone N81/37
merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody
merck millipore,默克密理博,506123,Anti-p38 MAP Kinase (341-360) Rabbit pAb
merck millipore,默克密理博,09-126,Anti-Tribbles-1 Antibody
merck millipore,默克密理博,05-621,Anti-CUGBP1 Antibody, clone 3B1
merck millipore,默克密理博,MAB880-A,Anti-HIV Antibody, p24, clone 7A8.1
%5BABT156_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,ABT156,Anti-CO-029 Antibody")
