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  • merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody

  • merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody

    产品名称：Anti-Bone Sialoprotein II Antibody
    产品型号：AB1854
    Anti-Bone Sialoprotein II Antibody is an antibody against Bone Sialoprotein II for use in ELISA, IC, IH, IH(P), RIA & WB.

    merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, HWB, ELISA, ICC, IHCRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB1854
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Bone Sialoprotein II Antibody
    Alternate Names
    • Integrin-binding Sialoprotein
    • BSPII
    背景信息 Bone sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers, Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling.
    产品信息
    格式 Serum
    演示 Lyophilized. Resuspend in 100 μL aqua bidest. Contains no preservatives.
    应用
    应用 Anti-Bone Sialoprotein II Antibody is an antibody against Bone Sialoprotein II for use in ELISA, IC, IH, IH(P), RIA & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Western Blot
    Immunohistochemistry (Paraffin) (see suggested protocol below)
    Immunocytochemistry (1:100)
    RIA
    ELISA
    Optimal working dilutions must be determined by the end user.

    SUGGESTED STAINING PROCEDURE FOR RABBIT ANTI-BONE SIALOPROTEINAB1854
    This protocol has been used successfully on 5 um, paraffin embedded sections from human fetal tibia, human fetal calvaria, human metastatic breast tumors to bone which were decalcified in 10% formic acid.

    1. Dewaxing: Xylene 5 min. - Xylene 5 min. - Xylene 5 min.
    2. Rehydration: 100% ethanol for 3 min. - 95% ethanol for 3 min. - 70% ethanol for 3 min.
    3. Wash sections under running tap water for 5 minutes.
    4. Wash sections in PBS.
    5. Block endogenous peroxidase: 0.3% hydrogen peroxide in absolute methanol (2 mL of H2O2 30% in 198 mL methanol) for 30 minutes. (Cover the jar with foil, use stirring during incubation.)
    6. Wash sections under running tap water for 10 minutes.
    7. Mark tissue area with pap pen.
    8. Cover the tissue area with 300 mL of blocking solution for 30 minutes at room temperature (blocking solution: 6 mL PBS, 0.3 gm BSA, 120 mL normal goat serum).
    9. Shake off excess fluid (do not wash).
    10. Incubate with primary antibody (AB1854 diluted 1:2,500 in PBS-T) at 2-8°C overnight in moist chamber.
    11. Rinse with PBS-T, then wash 3 times in PBS-T each for 5 minutes at room temperature.
    12. Incubate with biotinylated secondary antibody (for example Chemicon AP132B) for 30 minutes at room temperature.
    13. Rinse with PBS-T, then wash in PBS-T 3 x 5 minutes at room temperature.
    14. Incubate the sections with Vector ABC reagent for 30 minutes at room temperature. (ABC reagent: 2 drops of A and 2 drops of B, in 5 mL PBS, allow to stand 30 minutes at room temperature before use.)
    15. Wash sections in PBS 3 x 5 minutes at room temperature.
    16. Develop with DAB for 5-10 minutes.
    17. Wash under running tap water.
    18. Counter stain with Mayer's HX for 45 seconds, wash in warm tap water.
    19. Dehydrate: 70% ethanol - 95% ethanol - 100% ethanol, 1 min. each.
    20. Wash in Xylene, mount, dry.
    生物信息
    免疫原品种 Purified human Bone sialoprotein
    宿主 Rabbit
    特异性 Human and rat Bone sialoprotein (BSP). No cross reactivity with human osteopontin or osteonectin by RIA or ELISA.
    品种反应性 RatHuman
    Species Reactivity Note Human and rat. Other species have not been tested
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_004967.2
    Entrez基因汇总 The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
    基因符号
    • SP-II
    • IBSP
    • BNSP
    • BSP-II
    • BSP
    UniProt编号
    • P21815
    UniProt汇总 FUNCTION: SwissProt: P21815 # Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
    SIZE: 317 amino acids; 35018 Da
    SUBCELLULAR LOCATION: Secreted.
    PTM: N-glycosylated; glycans consist of sialated and core- fucosylated bi-, tri- and tetraantennary chains. & O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
    MISCELLANEOUS: It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C (lyophilized) or -20°C (reconstituted) for up to 6 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB1854,Anti-Bone Sialoprotein II Antibody

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