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品种反应性 主要应用 宿主 格式 抗体类型 B, H, R, M IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN34 描述 Anti-PDE11A Antibody Alternate Names - cAMP and cGMP phosphodiesterase 11A
- Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
背景信息 The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5’-AMP and 5’GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome. 产品信息 格式 Affinity Purified 控制 - Human hippocampus tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect PDE11A using this Anti-PDE11A Antibody validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE11A in normal mouse midbrain tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A. 表位 GAF domain. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes GAF domain of PDE11A. 品种反应性 BovineHumanRatMouse Species Reactivity Note Demonstrated to react with Human and Mouse. Predicted to react with Rat, and Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_058649
Entrez基因汇总 The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. 基因符号 - PDE11A
纯化方法 Affinity purified UniProt编号 - Q9HCR9
UniProt汇总 FUNCTION: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.
CATALYTIC ACTIVITY: Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.
ENZYME REGULATION: Inhibited by 3-isobutyl-1-methylxanthine (IBMX), zaprinast and dipyridamole. cGMP acts as an allosteric activator. Weakly inhibited by Sildenafil (Viagra) and Tadalafil (Cialis); however, the fact that the protein is probably absent from testis, suggests that it is not biologically relevant and is not related with erectile dysfunction.
SUBCELLULAR LOCATION: Cytoplasm > cytosol.
TISSUE SPECIFICITY: Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex.产品使用声明 质量保证 Evaluated by Western Blot in human hippocampus tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PDE11A in 10 µg of human hippocampus tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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