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Replacement Information Replacement Information 07-1401 is a recommended replacement for AB1340 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ELISA, WB, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1401 Replaces AB1340 描述 Anti-GLUT-1 Antibody, CT Alternate Names - Solute carrier family 2, facilitated glucose transporter, member 1
- Glucose Transporter type 1
- Solute carrier family 2
- facilitated glucose transporter
背景信息 GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose. 产品信息 格式 Affinity Purified 控制 - Jurkat Cell Lysate
演示 Purified rabbit polyclonal IgG in buffer containing 0.02 M Phosphate Buffer, 0.25 M NaCl, with 0.1% sodium azide. 应用 应用 Anti-GLUT-1 Antibody, C-terminus is an antibody against GLUT-1 for use in WB, IH(P), IC, ELISA. 主要应用 - ELISA
- Western Blotting
- Immunocytochemistry
应用说明 Immunohistochemisty (paraffin): GLUT-1 representative staining pattern/morphology on colorectal carcinoma. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. GLUT-1 immunoreactivity in erythrocytes.
Note: can be use as internal control for IHC run.
Immunocytochemistry: Recommended
ELISA: Recommended
Western Blot: A previous lot of this antibody was tested on multiple lysates using western blot analysis. See datasheet.生物信息 免疫原品种 Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV). 表位 C-Terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes GLUT-1. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006516
Entrez基因汇总 This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq].
基因符号 - MGC141896
- GLUT
- GLUT-1
- GLUT1
- MGC141895
纯化方法 Affinity Purfied UniProt编号 - P11166
UniProt汇总 FUNCTION: Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Melanosome. Note=Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: Expressed at variable levels in many human tissues.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: Defects in SLC2A1 are the cause of autosomal dominant GLUT1 deficiency syndrome [MIM:606777]. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily [view classification].
产品使用声明 质量保证 Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected GLUT-1 on 10 µg of Jurkat lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µg
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下一件merck millipore产品：merck millipore,默克密理博,ABC832,Anti-TMEM16B Antibody
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