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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB6057 描述 Anti-EB1 Antibody Alternate Names - APC-binding protein EB1
- microtubule-associated protein, RP/EB family, member 1
- adenomatous polyposis coli-binding protein EB1
- microtubule-associated protein RP/EB family member 1
- End-binding protein 1
背景信息 End binding protein 1 (EB1) is thought to be associated with adenomatous polyposis coli (APC) and may play a part in regulation of microtubule dynamics in the mammalian cells. EB1 could also play a role in the regulation of microtubule assembly and stability. EB1 is essential for proper cilia assembly in fibroblasts. EB1 has been shown to excite spontaneous nucleation and microtubule growth. APC gene mutation and the loss of negatively regulating WNT signaling is thought to play a role in the proliferation changes of cancer. The loss of association with EB1 could contribute to the development of certain cancers. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-EB1 Antibody is an antibody against EB1 for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected EB1 in NIH/3T3, A431, and HeLa cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of human EB1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes EB1 at the C-terminus.
EB1 is 67% homologous to EB3.品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human.
Predicted to react with mouse and rat based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036457
Entrez基因汇总 The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq]. 基因符号 - MAPRE1
- EB1
纯化方法 Protein A Purified UniProt编号 - Q15691
UniProt汇总 FUNCTION: May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
SUBUNIT STRUCTURE: Homodimer. Interacts with DCTN1, DCTN2, DIAPH1, DIAPH2, TERF1 and dynein intermediate chain. Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1. Interacts with CDK5RAP2.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › centrosome. Note: Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
TISSUE SPECIFICTY: Ubiquitously expressed.
DOMAIN: Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
SEQUENCE SIMILARITIES: Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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