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merck millipore,默克密理博,ECM551,QCM™ Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric
产品名称：QCM™ Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric
产品型号：ECM551
The Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells.
merck millipore,默克密理博,ECM551,QCM™ Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric
- 产品介绍
重要规格表
检测方法 Chromogenic 描述 产品目录编号 ECM551 品牌系列 Chemicon®
商名 - Chemicon
- QCM
描述 QCM™ Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric 概述 NEW!! High Sensitivity Collagen Invasion Assay! 24-well (8 µm), Colorimetric
Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!
Introduction
Penetration of the subendothelial basement membrane marks a critical turning point in the metastatic process. As proliferating neoplastic cells attempt to escape the primary tumor site, local invasion of the surrounding tissue (interstitial stroma) must occur. Neovascularization is initiated by expression of angiogenic factors (e.g. FGF, VEGF, HGF), providing nutritional requirements and access to the vascular system. Prior to penetrating the blood vessel endothelium and gaining access to the blood stream (intravasation), cancer cells must invade local tissues by degrading ECM components and ultimately, transverse the basement membrane. Once in circulation, these cells can form metastatic colonies at secondary locations, making this membrane a key invasive barrier.
The basement membrane surrounding the blood vessel endothelium is a thin, specialized network of extracellular matrix proteins (ECM) that serves many functions. Comprised of proteins and proteoglycans, such as collagen, laminin, entactin, fibronectin, heparin sulfate and perlecan, this membrane acts as a physical barrier between the epithelium and underlying tissues. It provides cell surface anchorage (via integrins, receptor kinases, and cell surface proteoglycans), induces cellular differentiation, gives architectural support, and limits the migration of normal cells. The ability of tumor cells to degrade the ECM components of the basement membrane and surrounding tissues is directly correlated with metastatic potential. By releasing proteolytic enzymes (e.g. MMP collagenases, plasminogen activators, cathepsins), cancer cells are able to breach the membrane and penetrate the blood vessel wall. Collagen, the primary structural element of the basement membrane and tissue scaffolding protein, represents the main deterrent in the migration of tumor cells.
The ability to study cell invasion through a collagen barrier, is of vital importance for developing possible metastatic inhibitors and therapeutics. The new CHEMICON QCM™ 24-well Collagen-based Invasion Assay (ECM551) provides an efficient, in vitro system for quantitative analysis of tumor cell invasion.
The CHEMICON QCM™ 24-well Collagen-based Invasion Assay (ECM551) eliminates cell pre-labeling and manual counting. The 24-well insert and colorimetric detection format allows for quantitative comparison of multiple samples. ECM551 uses purified chicken type I collagen as a matrix. The product is 99.9% pure native atelomeric avian collagen. Approximately 85% type I and 15% type III collagen protein. The material has been specially prepared to maintain its native state as much as possible. The telomeric ends of the collagen I have been enzymatically removed. Since most of the species identity is found within the telomeric ends, our atelomeric chicken collagen should provide a suitable substrate for most any animal species. The working concentration and precise purification protocol are proprietary and will not be disclosed, however it at least a 0.3% solution of treated avian collagen.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
· QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
· QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
· QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
· QCM™ 96-well Cell Invasion Assay (ECM555)
· QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)
· 24-well Insert Cell Migration and Invasion Assay Systems
· CytoMatrix™ Cell Adhesion strips (ECM protein coated)
· QuantiMatrix™ ECM protein ELISA kits
Test Principle
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert contains an 8 μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. The collagen layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the polymerized collagen layer and cling to the bottom of the polycarbonate membrane. Invaded cells on the bottom of the insert membrane are incubated with Cell Stain Solution, then subsequently extracted and detected on a standard microplate reader (560 nm).产品信息 部件 - Forceps: (Part No. 10203) One each.
- Sterile 24-well Cell Invasion Plate Assembly: (Part No. 90248) Two 24-well plates with 12 collagen-coated inserts per plate (24 inserts total/kit).
- Cell Stain: (Part No. 90144) One 20 mL bottle.
- Extraction Buffer: (Part No. 90145) One 20 mL bottle.
- Cotton Swabs: (Part No. 10202) 50 each.
检测方法 Chromogenic 应用 应用 The Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. 应用说明 The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 24 samples. The quantitative nature of this assay is especially useful for screening of pharmacological agents.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.生物信息 品种反应性 Vertebrates 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store kit materials at 2-8°C for up to their expiration date. Do not freeze. 包装信息 数量 24 wells 包装 24 wells
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